大鼠室旁核注射orexin-A对体重的影响  

Effect of Orexin-A Microinjected into the Paraventricular Nucleus of Hypothalamus on Body Weight of Rats

作　　者：杨丽媛[1,2] 孙姝[1] 逄明杰[3] 郭菲菲[1] 孙向荣[1] 公衍玲[4] 徐珞[1] 

机构地区：[1]青岛大学医学院病理生理教研室,山东青岛266021 [2]淄博市临淄区妇幼保健院,山东淄博255400 [3]青岛市立医院,山东青岛266000 [4]青岛科技大学,山东青岛266042

出　　处：《现代生物医学进展》2016年第8期1450-1453,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81470815;31071014;81100260;81270460;81300281;81500414);山东省优秀中青年科学家科研奖励基金项目(BS2014YY009);青岛市科技局项目(13-1-4-170-jch;14-2-3-3-nsh)

摘　　要：目的:探讨大鼠室旁核(PVN)注射orexin-A对体重的影响。方法:大鼠室旁核(PVN)微量注射orexin-A,用大脑置管埋管、组织化学染色等方法探讨PVN注射orexin-A对其体重的影响。结果:与安慰剂组大鼠相比,PVN注射orexin-A组大鼠体重明显减轻(P<0.05),而orexin-A组和安慰剂组摄食量无明显差异(P>0.05)。注射结束后6天,orexin-A处理大鼠的体重仍显著低于注射前(P<0.05),而安慰剂组大鼠则比注射前显著增重(P<0.05)。药物注射可显著降低机体脂肪,但并不特异存在于注射orexin-A或安慰剂的大鼠身上。Orexin-A组和安慰剂组大鼠的肌肉量和脂肪量均显著降低(P<0.05),但注射orexin-A的大鼠降低更明显。与安慰剂组相比,orexin-A处理后的摄食转化率显著降低(P<0.05)。结论:大鼠室旁核(PVN)注射orexin-A可通过增加活动量产生负能量平衡,引起体重减轻。To investigate the effect of orexin-A microinjected into the paraventricular nucleus of the hypothalamus（PVN） on body weight in rats. Methods： Chronic guide cannulae were implanted into PVN of rats. Orexin-A was microinjected into the brain site. The effect of orexin-A on body weight in rats was detected. Results： The loss of body weight in orexin-A-treated rats was more significantly than their vehicle-injected counterparts（P〈0.05）, but they had no difference in food intake（P〈0.05）. The rats were more significantly active after intra-PVN orexin microinjection compared to vehicle（P〈0.05）. Conclusion： Orexin-A microinjected into the paraventricular nucleus of the hypothalamus（PVN） can induce weight loss in rats through producing negative energy balance via the increase of physical activity.

关 键 词：OREXIN-A PVN 非运动性活动产热 负能量平衡 体重 

分 类 号：R338.2[医药卫生—人体生理学] R589.2[医药卫生—基础医学]