消癌解毒方含药血清对人肝癌SMMC-7721细胞端粒酶及凋亡相关基因mRNA表达的影响  

Effects of Xiao'ai Jiedu Formula drug-containing serum on telomerase and apoptosisrelated mRNA expression in human hepatoma SMMC-7721 cells

作　　者：李文婷[1] 赵凤鸣[1] 周红光[1] 周韬[1] 李沐涵[1] 李黎[1] 吴勉华[1] 

机构地区：[1]南京中医药大学,江苏省中医药防治肿瘤协同创新中心,南京210023

出　　处：《中华中医药杂志》2016年第4期1211-1214,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81273717,No.81473608,No.81373511);国家自然科学基金青年项目(No.81102563);中华人民共和国教育部课题(No.20113237110001);江苏省普通高校研究生科研创新计划省立项目(No.CXLX12_0572)~~

摘　　要：目的:探讨消癌解毒方含药血清对人肝癌细胞SMMC-7721细胞端粒酶及凋亡相关基因mRNA表达的影响。方法:消癌解毒方含药血清作用于人肝癌SMMC-7721细胞,ELISA试剂盒检测各组端粒酶浓度,采用实时荧光定量PCR方法检测药物作用前后各组SMMC-7721细胞Bax、Bcl-2、CytC、Caspase-3 mRNA表达水平。结果:消癌解毒方含药血清作用于人肝癌SMMC-7721细胞可以降低其端粒酶浓度,且呈剂量依赖性;可使Bcl-2 mRNA表达水平下调,使Bax、CytC、Caspase-3 mRNA表达水平上调,下调Bcl-2/Bax mRNA比例,且中、高剂量组与对照组比较效果明显(P<0.01,P<0.05)。结论:消癌解毒方含药血清可能通过抑制端粒酶活性,调控凋亡相关基因mRNA的表达,抑制人肝癌SMMC-7721细胞生长,促使其凋亡,在肝癌治疗中发挥疗效。Objective： To study the effects of Xiao＇ai Jiedu Formula drug-containing serum on telomerase and apoptosisrelated mRNA expression in human hepatoma SMMC-7721 cells. Methods： The effects of Xiao＇ai Jiedu Formula drug-containing serum on human hepatoma SMMC-7721 cells was observed. The concentration of telomerase in each groups were measured by ELISA kits, and the expression of Bax, Bcl-2, CytC and Caspase-3 mRNA in SMMC-7721 cells were measured by Real Time PCR method before and after medication. Results： Xiao＇ai Jiedu Formula drug-containing serum could reduce the telomerase concentration of human hepatoma SMMC-7721 cells and the efficacy was dose-dependent. It was also showed that Xiao＇ai Jiedu Formula can down-regulate the Bcl-2 mRNA expression, up-regulate the expression of Bax, CytC, Caspase-3 mRNA, and decrease the Bcl-2/Bax mRNA ratio. The efficacy of Xiao＇ai Jiedu Formula was more obvious in the middle and high-dose groups than in the control group（P〈0.01, P〈0.05）. Conclusion： Xiao＇ai Jiedu Formula is able to inhibit the growth of human hepatoma SMMC-7721 cells and promote the apoptosis by suppressing telomerase activity and regulating the expression of apoptosis-related mRNA, thus playing a part in treating liver cancer.

关 键 词：消癌解毒方 人肝癌SMMC-7721细胞 端粒酶 实时荧光定量PCR 凋亡 Bax Bcl-2 CYTC Caspase-3 

分 类 号：R285[医药卫生—中药学]