慈济化癌保生汤对H22肝癌荷瘤化疗模型小鼠外周血细胞与脾脏造血因子的影响  

Effects of Ciji Hua'ai Baosheng Decoction on peripheral blood cells and spleen hematopoietic growth factors of tumor chemotherapy model mice with H22 hepatoma carcinoma cells

作　　者：程尧[1] 奚胜艳[1] 王彦晖[1] 史萌萌[1] 刘培[1] 李鹏程[1] 

机构地区：[1]厦门大学医学院中医系,厦门361102

出　　处：《中华中医药杂志》2016年第4期1245-1248,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学青年基金项目(No.81202659);厦门市重大科技计划项目(No.3502Z20100006);福建省自然科学基金项目(No.2014J01373);厦门市科技计划项目(No.3502Z20153027)~~

摘　　要：目的:研究慈济化癌保生汤(CHBT)对H22肝癌化疗小鼠外周血细胞以及脾脏造血生长因子红细胞生成素(EPO)、粒细胞-巨噬细胞集落刺激因子(GM-CSF)以及造血功能的作用。方法:将肝癌H22细胞悬液(2×107/m L)接种于Kunming种小鼠右前腋皮下,约7d后,全部形成移植瘤,环磷酰胺(CTX)按200mg/kg(0.2m L/10g)腹腔注射,建立H22肝癌小鼠化疗模型。随机分为模型组,CTX(0.033g/kg)组,CHBT高、中、低剂量(117、58.5、29.25g/kg)组,连续给药10d,采用全自动血细胞分析仪检测外周血红细胞、白细胞、血小板和血红蛋白,运用酶联免疫吸附测定法检测脾组织EPO和GM-CSF含量。结果:与模型组和CTX组比较,CHBT高、中、低剂量组红细胞、白细胞、血小板数量明显升高(P<0.05,P<0.01);CHBT高、中剂量组血红蛋白升高(P<0.05,P<0.01);CHBT各剂量组EPO、GM-CSF含量升高(P<0.01,P<0.05)。结论:慈济化癌保生汤能促进化疗后EPO和GM-CSF的生成并增强活性,刺激血细胞的产生,维持外周血象稳定性。Objective： To investigate the effects of Ciji Hua＇ai Baosheng Decoction（CHBT） on peripheral blood cells, spleen hematopoietic growth factors such as erythropoietin（EPO） and granulocyte-macrophage colony stimulating factor（GM-CSF）, and blood-producing function of tumor chemotherapy model mice with H22 hepatoma carcinoma cells. Methods： The suspension of H22 hepatoma carcinoma cells（2×107/m L） were subcutaneously injected into the right anterior armpit of Kunming mice. After 7 days, all mice formed the transplanted tumors, and then the cytoxan（CTX） at the dosage of 200mg/kg were intraperitoneally injected into the mice to establish the tumor chemotherapy model. The mice were randomly divided into model group, CTX（0.033g/kg） group, CHBT high, middle and low dose（117, 58.5, 29.25g/kg） groups. The experiment groups were treated for 10 days. The levels of red blood cells, white blood cells, platelet and hemoglobin in peripheral blood were detected by automatic blood cell analyzer. The contents of EPO and GM-CSF in spleen tissue were assayed by enzyme-linked immunosorbent assay. Results： Compared with model group and CTX group, the levels of red blood cells, white blood cells and platelet in high, middle and low dose CHBT groups were increased significantly（P〈0.05, P〈0.01）, the levels of hemoglobin in CHBT high and middle dose groups were increased（P〈0.05, P〈0.01）, the contents of EPO and GM-CSF in CHBT high, middle and low dose groups were increased significantly（P〈0.05, P〈0.01）. Conclusion： The CHBT in given concentration could promote the production of EPO and GM-CSF after tumor chemotherapy and stimulate the hemocytogenesis to maintain the stability of peripheral haemogram, which may be the possible mechanism of CHBT ameliorating the haemogram of cancer after chemotherapy.

关 键 词：慈济化癌保生汤 化疗 移植瘤 外周血细胞 促红细胞生成素 粒细胞-巨噬细胞集落刺激因子 

分 类 号：R285.5[医药卫生—中药学]