艾灸及艾烟对动脉粥样硬化模型小鼠血清TNF-α、hs-CRP及vWF的影响  

Effects of moxibustion and moxa smoke on TNF-α, hs-CRP, vWF in serum of atherosclerosis mice

作　　者：刘耀萌 崔莹雪[2] 哈略 赵百孝[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]首都医科大学附属北京中医医院针灸中心,北京100010

出　　处：《中华中医药杂志》2016年第4期1377-1379,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点基础研究发展计划(973计划)项目(No.2009CB522906);国家自然科学基金面上项目(No.81373730);国家自然科学基金青年基金项目(No.81403449);北京中医药大学自主课题项目(No.2015-JYB-XS125)~~

摘　　要：目的:通过研究艾灸及艾烟干预对动脉粥样硬化模型小鼠(ApoE^(-/-)小鼠)血清肿瘤坏死因子α(TNF-α)、超敏C反应蛋白(hs-CRP)、血管假性血友病因子(vWF)含量的影响,探讨艾灸及艾烟的作用机制。方法:将51只8周龄ApoE^(-/-)小鼠随机分为3组:模型组、艾烟组、艾灸组,每组17只,20只同月龄C57BL/6小鼠作为空白对照。艾灸柱灸治小鼠关元穴,艾烟组小鼠暴露于10-15mg/m3艾烟环境;正常组、模型组小鼠常规抓取、束缚于正常环境。所有动物干预20min/d,6d/周,连续干预12周。于第13周取材,ELISA法检测血清中TNF-α、hs-CRP、vWF含量。结果:血清中TNF-α、hs-CRP、vWF含量,模型组显著高于正常组(P<0.01);艾灸组、艾烟组显著低于模型组(P<0.01)。艾灸组小鼠各项指标与艾烟组无显著差异。结论:艾灸及艾烟可通过影响ApoE^(-/-)小鼠血清TNF-α、hs-CRP、vWF含量,降低动脉粥样硬化病变过程中的炎性反应,改善动脉粥样硬化病的预后和转归。Objective： To explore effects of moxibustion and moxa smoke on TNF-α, hs-CRP, vWF in serum of atherosclerosis mice with atherosclerosis model. Methods： A total of 51 ApoE^（-/-） mice, 8-week-old, were ramdomly divided into three groups： model group, moxibustion group, and moxa smoke group, with 17 mice in each group. And, twenty C57BL/6 mice were choosed as the control group. Moxibustion was performed with a moxa stick on Guanyuan（CV4） in moxibustion group, while mice in moxa smoke group were exposed to a moxa smoke environment of 10-15mg/m3. The mice in normal and model groups were routinely grabed and bound in a normal environment. All inventions lasted for 20min/d, 6d/week, and continuously intervened for 12 weeks. All mice were sacrificed in the 13 th weeks. The contents of TNF-α, hs-CRP, and vWF in serum were tested with ELISA. Results： The levels of TNF-α, hs-CRP and vWF in the model group were much higher than those of the normal group（P〈0.01）, while the moxibustion and moxa smoke groups were lower than the model group（P〈0.01）. There was no significant difference in each indicator between the moxibustion group and the moxa smoke group. Conclusion： Moxibustion and moxa smoke could reduce the inflammatory reactions in the process of atherosclerosis by influencing the levels of TNF-α, hsCRP and vWF, as a result of an improvement of atherosclerosis.

关 键 词：动脉粥样硬化 艾灸 艾烟 肿瘤坏死因子α 超敏C反应蛋白 血管性血友病因子 

分 类 号：R245[医药卫生—针灸推拿学] R-332[医药卫生—中医临床基础]