蜘蛛香环烯醚萜类成分ethoxyviburtinal-11对大鼠结肠纵行肌细胞收缩效应的影响  

Effects of ethoxyviburtinal-11 on the contractile response of rat's colon longitudinal muscle cells and its mechanism

作　　者：张婷[1,2] 胡京红[1] 王晶[1] 闫兴丽[1] 王娟[1] 罗武政[1] 施金钹 陶丝雨 

机构地区：[1]北京中医药大学,北京100029 [2]北京市延庆县食品药品安全监控中心,北京102100

出　　处：《中华中医药杂志》2016年第4期1386-1389,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81073132;No.81374044);北京中医药大学自主课题(No.2011YJB22X5029)~~

摘　　要：目的:研究蜘蛛香环烯醚萜类成分ethoxyviburtinal-11对大鼠结肠纵行肌细胞的直接作用及初步机制探讨。方法:原代培养大鼠结肠纵行肌细胞,观察不同时间、不同浓度的ethoxyviburtinal-11对大鼠结肠纵行肌细胞的作用及ethoxyviburtinal-11对工具药5-羟色胺(5-HT)、乙酰胆碱(Ach)和去甲肾上腺素(NE)引起结肠纵行肌细胞收缩效应的影响。结果:当浓度为4.04mmol/L,作用30s时,ethoxyviburtinal-11对结肠纵行肌细胞具有最强的舒张效应。ethoxyviburtinal-11对5-HT引起的结肠纵行肌细胞收缩效应有显著抑制作用,而对Ach、NE的效应无显著性影响。结论:ethoxyviburtinal-11对结肠纵行肌细胞具有直接的舒张作用,其舒张作用可能与5-HT有关。Objective： To observe the effects of ethoxyviburtinal-11, a constituent of valeriana jatamansi jones, on rats＇ colon longitudinal muscle cells and its mechanism. Methods： The primary cultured rat colon longitudinal muscle cells were administrated by ethoxyviburtinal-11 at different time and concentration. The shrinkage effect induced by tool medicines including serotonin（5-HT）, acetylcholine（Ach） and norepinephrine（NE） was observed after the administration of ethoxyviburtinal-11. Results： Ethoxyviburtinal-11 had a strongest relaxed effect on primary cultured rat colon longitudinal muscle cells when the concentration was 4.04mmol/L and the time was 30 s. Ethoxyviburtinal-11 could significantly inhibit the shrinkage effect induced by 5-HT but Ach and NE. Conclusion： The ethoxyviburtinal-11 could directly make the rats＇ colon longitudinal muscle cells relax, which may be related to 5-HT.

关 键 词：蜘蛛香 ethoxyviburtinal-11 大鼠结肠纵行肌细胞 细胞长度变化百分率 肠易激综合征 

分 类 号：R285.5[医药卫生—中药学]