不同产地栽培及野生喜马拉雅紫茉莉的分子鉴定研究  

Molecular identification of wild and cultivated Mirabilis himalaica in different districts

作　　者：林辉[1] 牟银艳 赵婷[3] 仁青加[4] 李佳慧[3] 彭莲[3] 闫永红[3] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030 [2]利川市民族中医院,湖北利川445400 [3]北京中医药大学,北京100029 [4]西藏藏医学院,拉萨850000

出　　处：《中华中医药杂志》2016年第4期1427-1429,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：北京市青年英才计划(No.50010402/0101324003)~~

摘　　要：目的:利用分子技术测定喜马拉雅紫茉莉的DNA分子序列,比较不同产地栽培及野生喜马拉雅紫茉莉的差异,为栽培品的合理使用提供依据。方法:提取喜马拉雅紫茉莉药材的DNA,以ITS序列通用引物进行PCR扩增,扩增产物经纯化后测序,利用NCBI数据库及MEGA 5.0等软件对数据进行分析。结果:喜马拉雅紫茉莉样品间DNA遗传距离差异较小,在0.000-0.040之间;样品分子间碱基变异位点少;系统发育树中,所有样品聚为一大类,Boerhavia spicata.聚为一类,二者显著分开。而安国XZAGZP及甘南州GSGLYS聚为一类,其余样品聚为一类。结论:不同产地栽培及野生的喜马拉雅紫茉莉药材在DNA分子序列方面的差异较小,为栽培品的合理使用提供了理论依据。Objective： To compare the differences of wild and cultivated Mirabilis himalaica in different districts by molecular techniques and offer the basis for the use of cultivated Mirabilis himalaica. Methods： DNA was extracted from wild and cultivated Mirabilis himalaica, then PCR with ITS universal primers. The PCR products were purified, then directly sequenced. In the end, the data were analyzed by NCBI database and MEGA 5.0 software. Results： The genetic distance in Mirabilis himalaicafrom different habitats ranged from 0.000 to 0.040. The variable sites was small. The phylogenetic trees suggested that there were close genetic relationship among Mirabilis himalaica. XZAGZP and GSGLYS were clustered into one group, which comprised another clade. Conclusion： There is no significant difference in the variability of Mirabilis himalaica from different districts, which is a foundation for the rational use of cultivated materials.

关 键 词：喜马拉雅紫茉莉 分子鉴定 ITS序列 系统发育树 

分 类 号：S567.19[农业科学—中草药栽培]