外源茉莉酸甲酯诱导小麦抗条锈病的研究  

Preliminary study on resistance of wheat to stripe rust induced by methyl jasmonate

作　　者：蔚慧欣 高利[2] 刘太国[2] 刘博[2] 康晓慧[1] 陈万权[2] 

机构地区：[1]西南科技大学生命科学与工程学院,绵阳621010 [2]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《植物病理学报》2016年第2期190-197,共8页Acta Phytopathologica Sinica

基　　金：973计划(2013CB127701;2011CB100403);小麦产业技术体系(CARS-03);北京市科技新星计划(Z131105000413057)

摘　　要：本文探讨了不同浓度茉莉酸甲酯(MeJA)对小麦抗病性的诱导作用,同时电镜观察了不同浓度MeJA对小麦条锈菌(Puccinia striiformis f.sp.tritici)与寄主细胞超微结构的影响。结果表明,2.00 mmol·L^(-1) MeJA对小麦条锈病的诱导抗性效果最好,可达到35.87%,而0.50 mmol·L^(-1)MeJA在小麦条锈菌夏孢子开始萌发4 h时呈显著抑制作用。经外源MeJA处理后,条锈菌的胞间菌丝、吸器母细胞和吸器的发育明显受到抑制,其中侵染位点的叶肉细胞呈抗性症状。因此,MeJA可使小麦幼苗产生诱导抗性从而提高其抗病性。To understand the effects of different concentrations of methyl jasmonate（ MeJA） on the resistance of wheat seeding against stripe rust caused by Puccinia striiformis f. sp. tritici（ Pst）,wheat seedling was inoculated with Pst after spraying with different concentrations of MeJA. The inducing effect was observed in the resistance to stripe rust pretreated with MeJA at the concentration of 2. 00 mmol·L^（-1） with the effect reached to35. 87%,MeJA could inhibit the germination of urediniospore significantly at the concentration of 0. 50 mmol·L^（-1） after 4 hours inoculation. Ultrastructure of intercellular hyphae,haustorium,and haustorial mother cells were significantly inhibited. A series of changes associated with resistance to infection were reported. Our results indicated that the resistance of wheat seedling against Pst was induced after MeJA treatment.

关 键 词：茉莉酸甲酯 小麦条锈病 诱导抗性 超微结构 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治] S512.1[农业科学—植物保护]