四川水稻抗稻曲病种质资源筛选和多态性分析（英文）  

Screening and Polymorphism Analysis of Rice Germplasms for Resistance to False Smut Disease in Sichuan Province

作　　者：黄富[1] 李燕[2] 石军[2,1,3] 樊晶[2] 李德强[1] 徐永菊[2] 王文明[2] 

机构地区：[1]四川农业大学植物病理系,成都611130 [2]四川农业大学水稻研究所,成都611130 [3]四川省绵阳市农业科学研究院,绵阳621023

出　　处：《植物病理学报》2016年第2期247-257,共11页Acta Phytopathologica Sinica

基　　金：Agro-scientific Research in the Public Interest(200903039-7)

摘　　要：稻曲病近年来逐步上升为世界多个水稻产区的主要病害之一。利用丰富的抗性种质资源进行抗性品种选育是控制稻曲病害的重要手段。通过在四川省进行三年病圃筛选试验,我们从843份水稻材料中鉴定出179份完全不感稻曲病的抗性材料和两份高感材料（病穗率高达50%以上）,其余材料的病穗率介于0.1%~48.8%之间,55.1%的病穗只有一个稻曲球,而高感稻曲病的蒲江6号单穗稻曲球高达38粒。然后我们选取36份材料进行多点多期播种,进一步验证其稻曲病抗感性。结果表明,有3份材料在所有试验中都不感稻曲病,18份材料偶发稻曲病呈现高抗,蒲江6号仍然表现为高感。我们用450对SSR引物对其中35份材料与蒲江6号进行多态性分析,发现其中12份材料与蒲江6号的多态性达10%以上,其中最高为ITAT144（16.1%）,其次是泸香90-2（14.8%）和Domsia-2（14.4%）。因此,我们选择这些材料与蒲江6号杂交构建基因定位与克隆群体。Rice false smut disease is an increasingly concerned problem in both rice research and production.Diverse resistance germplasm is the most important factor in resistance breeding program for controlling this disease.After three years of screening in our disease nursery in Sichuan Province,out of 843 accessions we identified179 that showed no occurrence of false smut disease and two accessions that were highly susceptible with diseased panicle percentage more than 50%. The rest accessions had diseased panicle percentage ranging from0. 1% to 48. 8%. More than half（ 55. 1%） of the infected panicles formed only one smut ball,but the number reached up to 38 in the most susceptible accession Pujiang 6. Then we selected 36 accessions for further evaluation of disease severity variation at two locations with 2-3 planting times with Pujiang 6 as a susceptible control.Three of the 36 accessions showed no disease in all these experiments,and 18 accessions were occasionally infected and thus were highly resistant to false smut disease. In order to find accessions with the highest polymorphism to the susceptible accession Pujiang 6,we performed polymorphism analysis between 35 accessions and Pujiang 6 by using 450 simple sequence repeat（ SSR） markers. Twelve accessions showed polymorphism to Pujiang6 in more than 10% of these markers. IRAT144 was at the first place with 16. 1% of polymorphism rating,followed by Luxiang 90-2 and Domsia-2 with 14. 8% and 14. 4%,respectively. Thus the 12 accessions were selected to cross with Pujiang 6 to construct gene mapping and cloning populations.

关 键 词：水稻 稻曲病 抗性 多态性 种质资源 

分 类 号：S828[农业科学—畜牧学]