HPLC法测定复方甲氧沙林注射液中甲氧沙林的含量  

Determination of Methoxsalen in Compound Methoxsalen Injection by HPLC

作　　者：刘春光[1] 姜静岩[2] 陈新善[3] 

机构地区：[1]武警北京总队医院,北京100027 [2]解放军251医院,河北张家口075000 [3]总后勤部卫生部药品仪器检验所,北京100166

出　　处：《解放军药学学报》2016年第1期55-56,59,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立HPLC法测定复方甲氧沙林注射液中甲氧沙林的含量。方法 HPLC法,采用CAPCELL PAK C18UG120色谱柱（Dimensions 4.6 mm I.D.×250 mm,5μm）,乙腈-水（35∶65）为流动相,检测波长：248 nm;流速：1.0 ml·min-1;柱温：30℃。结果甲氧沙林在1.025～10.25μg·ml-1的范围内线性关系良好（r=1.0000）,平均回收率为99.68%,RSD为0.54%（n=9）。结论本方法简便、测定结果准确,重复性好,可用于复方甲氧沙林注射液中甲氧沙林的含量测定。Objective To establish an HPLC method for determination of methoxsalen in compound methoxsalen injection. Methods CAPCELL PAK C18UG120 column（ Dimensions 4. 6 mm I. D. × 250 mm） was used as the chromatographic column,and the mobile phase was composed of acetonitrile-water（ 35∶ 65）. The flow rate was 1. 0 ml·min- 1and detection wavelength was 248 nm. The column temperature was 30 ℃. Results The calibration curve of methoxsalen showed a good linear relationship over the range of 1. 025- 10. 25 μg·ml- 1（ r = 1. 0000）. The average recovery of methoxsalen was 99. 68% and RSD was 0. 54%（ n = 9）. Conclusion This method is simple,accurate and with good reproducibility. It can be used for the determination of methoxsalen in compound methoxsalen injection.

关 键 词：复方甲氧沙林注射液 甲氧沙林 HPLC 含量测定 

分 类 号：R927.2[医药卫生—药学]