鹿茸草对脂多糖诱导的大鼠ALI的保护作用研究  

Monochasma savatieri attenuates lipopolysaccharide-induced acute lung injury in rat

作　　者：李明明[1] 张卫东[1] 王荣[2] 姜涛[3] 金发光[4] 李建忠[5] 李晓平[1] 郭健[1] 王旭[1] 周琳[3] 张鸿[3] 杨波[5] 

机构地区：[1]天津市第一中心医院胸外科,300192 [2]西安交通大学航天航空学院,710049 [3]第四军医大学唐都医院胸外科,西安710038 [4]第四军医大学唐都医院呼吸科 [5]哈尔滨医科大学附属第二医院心血管外科,150001

出　　处：《国际呼吸杂志》2016年第4期288-291,共4页International Journal of Respiration

基　　金：国家自然科学基金，天津市卫生局面上项目

摘　　要：目的 探讨鹿茸草对脂多糖（LPS）诱导的大鼠ALI的保护作用。方法健康成年雄性SD大鼠60只随机分为4组：对照组、鹿茸草组、LPS组、LPS＋鹿茸草组，每组15只。LPS诱导肺损伤前30min，鹿茸草水煎液0．2g／ml和生理盐水分别自胃内注射到大鼠体内。大鼠ALI通过气管滴注LPS（3mg／kg）诱导。2h后麻醉处死大鼠，评估肺损伤情况。结果预先给予鹿茸草，可使肺泡出血和炎性损伤表现相对减轻，肺组织湿干重比值明显降低。此外，鹿茸草显著抑制炎症因子IL-1β和肿瘤坏死因子a的表达水平以及细胞凋亡指数。结论鹿茸草对LPS诱导的大鼠ALI具有肺保护作用。Objective To analyze the role of Monochasma savatieri in acute lung injury induced by lipopolysaccharide （LPS） in rat. Methods Sixty male SD rats were randomly divided into four groups （15 in each group）： control group, Monoehasma savatieri group （0.2 g/ml）, I.PS group, and LPS＋Monochasma savatieri group. Monochasma savatieri or NaCI was given with intragastric administration 30 rain before intratracheal instillation of LPS （3 mg/kg）. The severity of pulmonary injury was evaluated 2 h after LPS induction. Results Monochasma savatieri pretreatment significantly attenuated LPS-induced pulmonary histopathologic changes, alveolar hemorrhage, and neutrophil infiltration. The lung wet-to-dry weight ratio, as the index of pulmonary edema, was markedly decreased by Monochasma savatieri pretreatment. Moreover, Monochasma savatieri decreased the production of the proinflammatory cytokines including tumor necrosis factor-a, interleukin-1β and apoptosis index. Conclusions Monochasma savatieri has protective effects on LPS-induced acute lung injury in rat.

关 键 词：鹿茸草 脂多糖 急性肺损伤 肿瘤坏死因子A 白介素1Β 

分 类 号：R285.5[医药卫生—中药学]