长筒石蒜组织培养中器官发生的MSAP分析  

MSAP analysis of Lycoris longituba organogenesis in tissue culture

作　　者：刘合霞[1] 李博[2] 周坚[3] 

机构地区：[1]广西壮族自治区中国科学院广西植物研究所园林园艺研究中心,广西桂林541006 [2]桂林师范高等专科学校化学与药学系,广西桂林541001 [3]南京林业大学生物与环境学院,江苏南京210037

出　　处：《中山大学学报（自然科学版）》2016年第2期102-110,共9页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广西植物研究所基本科研业务费资助项目(桂植业14003);国家林业公益性行业科研专项资助项目(201004056);江苏省研究生培养创新工程资助项目(CXZZ12-0519)

摘　　要：采用甲基化敏感扩增多态性技术,从64对MSAP选扩引物中,选出扩增清晰、可辨且可重复的51对MSAP引物组合,扩增获得长筒石蒜种子胚、愈伤组织、苗等3个不同发育时期的DNA胞嘧啶甲基化修饰水平及MSAP扩增图谱。结果表明,种子胚基因组中有68.0%(全甲基化50.2%、半甲基化49.8%)、愈伤组织60.0%(全甲基化52.2%、半甲基化47.8%)、苗64.3%(全甲基化40.8%、半甲基化59.2%)的CCGG/GGCC位点发生胞嘧啶甲基化。3个不同时期甲基化的水平互有差异,依据超甲基化/去甲基化的修饰机理分析,甲基化水平的变化或许导致了长筒石蒜不同的器官发生。In this study,DNA cytosine methylation modification level and MSAP amplification graphs of Lycoris longituba were obtained from seed embryo,callus and seedling via methylation sensitive amplification polymorphism technology. We found that 51 pairs of MSAP selective amplifying primer combinations were good in 64 pairs of the MSAP selective amplifying primer combinations. The results showed that the cytosine methylation rates were 68. 0% in seed embryo（ full methylation： 50. 2% and half methylation：49. 8%）,60. 0% in callus（ full methylation： 52. 2% and half methylation： 47. 8%） and 64. 3% in seeding（ full methylation： 40. 8% and half methylation： 59. 2%）. The level of methylation differed with each other in the three different statges. So,it is suggested that changes of the level of methylation may had led to their different organs of Lycoris longituba based on the modification mechanism of super methylation / demethylation analysis.

关 键 词：长筒石蒜 MSAP 器官发生 

分 类 号：Q78[生物学—分子生物学]