连作土灭菌对葡萄根际土壤微生物群落功能的影响  

Effects of sterilization of continuous mono-cropping soil on microbial community function in rhizosphere soil of grapevines

作　　者：李坤[1] 朱绍坤 郭修武[1] 谢洪刚[2] 郭印山[1] 刘镇东[1] 

机构地区：[1]沈阳农业大学园艺学院,沈阳110866 [2]辽宁经济管理干部学院,沈阳110112

出　　处：《生态学杂志》2016年第4期972-976,共5页Chinese Journal of Ecology

基　　金：现代农业产业技术体系专项基金项目(CARS-30-yz-06);国家自然科学基金项目(31101503);辽宁省科技厅重大科技攻关项目(2011204001);教育部高校博士点专项资金项目(20112103120012)资助

摘　　要：为了明确土壤微生物群落功能与植株长势之间的相关性,本试验以种植葡萄30年的连作土为研究对象,研究不同温度灭菌对葡萄植株生长及根际微生物群落功能的影响。结果表明:种植在连作土中的葡萄株高及茎粗低于其他处理;随灭菌温度的增加,葡萄长势增强;葡萄根际土壤细菌/真菌比值随着连作土壤灭菌温度的升高而增加,根际土壤微生物利用碳源的活性呈现100℃灭菌连作土>正常土>60℃灭菌连作土>连作土的规律。回归分析结果表明,ECO平板中碳源氨基酸与根际土壤微生物代谢活性具有显著相关性。主成分分析表明,根系分泌物中赖氨酸对各处理主成分方差贡献率最大,其可能在葡萄连作障碍中发挥着重要作用。In order to explore the correlation between soil microbial community function and plant growth,using 30-year continuous cropping soil of grapevines as research object,we studied the effects of sterilization of continuous cropping soil at different temperatures on the growth of grapevines and the microbial community function of rhizosphere soil. The results showed that plant height and stem diameter of grapevines grown in continuous cropping soil were lower than those in the other treatments. With the increasing sterilization temperature,the plant height and stem diameter of grapevines increased. The ratio of bacteria to fungi in rhizosphere soil increased with the increasing sterilization temperature. The activity of rhizosphere microbes using carbon source was in order of continuous cropping soil sterilized at 100 ℃ 〉non-continuous cropping soil continuous cropping soil sterilized at 60 ℃ 〉continuous cropping soil. The regression analysis showed that amino acid（ carbon source） in Biolog ECO-plate had a significant correlation with microbial metabolic activity of rhizosphere soil. PCA analysis showed that lysine in root exudate had a highest contribution to the variance of principal components in each treatment,and it may play an important role in the obstacle of continuous cropping of grapevines.

关 键 词：根际微生物 种群结构 代谢活性 根系分泌物 氨基酸 

分 类 号：S154.3[农业科学—土壤学] S663.1[农业科学—农业基础科学]