Parthenogenetic haploid embryonic stem cells efficiently support mouse generation by oocyte injection  

作　　者：YuxuanWu Li Yang Jinsong Li Cuiqing Zhong Zhenfei Xie Qi Yin Rui Dong Suming Yang 

机构地区：[1]Group of Epigenetic Reprogramming, State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China [2]Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Scienc- es, Chinese Academy of Sciences, Shanghai 200031, China [3]University of Chinese Academy of Sciences, Beijing 100049, China [4]Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China

出　　处：《Cell Research》2016年第1期131-134,共4页细胞研究（英文版）

基　　金：We thank Wei Bian, Xuedong Wang, and Fang Zhang for support with cell sorting and Drs Yuelei Chen and Xiaoyan Ding from the Stem Cell Core Facility at SIBCB for support with cell cultures. This study was supported by the Ministry of Science and Technology of China （2014CB964803, 2015AA020307 and 2013CB967103）, the National Natural Science Foundation of Chi- na （91319310 and 31225017）, the Chinese Academy of Sciences （XDA01010403）, and the Shanghai Municipal Commission for Science and Technology （12JC1409600 and 13XD1404000）.

摘　　要：Dear Editor, Mammalian haploid embryonic stem cells （haESCs） have been recently generated from parthenogenetic and androgenetic embryos [1, 2]. Both parthenogenetic haESCs （PG-haESCs） and androgenetic haESCs （AGhaESCs） can be used for cell-based reverse and forward genetic screens on a whole-genome scale [3, 4]. AGhaESCs, after intracytoplasmic injection into oocytes （referred to as CAHCI）, can be used as a sperm replacement to produce healthy semi-cloned （SC） mice at a rate of-2% of transferred embryos [5, 6].

关 键 词：卵胞浆内单精子注射 胚胎干细胞 卵母细胞 单倍体 鼠标 哺乳动物 孤雌生殖 全基因组 

分 类 号：Q492[生物学—生理学] Q813