5年生种植人参对小鼠学习记忆的影响  

The effect of Ginseng-5year on learning and memory in mice

作　　者：谢湘林[1] 刘蕾[1] 谢宇[2] 方圣博 杜冰[1] 睢大筼[1] 刘宏雁[1] 

机构地区：[1]吉林大学药学院药理教研室,长春130021 [2]吉林大学口腔医院修复科,长春130021

出　　处：《人参研究》2016年第1期2-5,共4页Ginseng Research

基　　金：吉林省重大科技攻关项目;项目编号:10ZDGG013

摘　　要：目的观察5年生种植人参原粉、水提物和醇提物长期应用对小鼠学习记忆的影响,为人参的食用提供实验依据。方法正常成年昆明小鼠140只,雌雄各半,体重18~22g,随机分为10组,即：对照组,5年生种植人参原粉小、中、大剂量（0.25、0.5、1.0g生药/kg）组,5年生种植人参水提物小、中、大剂量（0.25、0.5、1.0g生药/kg）组,5年生种植人参醇提物小、中、大剂量（0.25、0.5、1.0g生药/kg）组,每组14只,连续灌胃给药3个月后进行东莨菪碱致学习记忆获得障碍实验。结果与模型组比较,人参醇提物大剂量小鼠第1天的到达平台潜伏期、游程有缩短的趋势,但无统计学意义,第4天的游程明显缩短（P〈0.05）,其他指标无明显变化。人参原粉小、中、大剂量组,人参水提物小、中、大剂量组,人参醇提物小、中剂量组第1天至第5天到达平台的潜伏期、游程、朝向角、游泳速度无明显变化,第6天小鼠在1.5min内穿越平台的次数、穿越有效区的次数、平台停留距离、平台区的逗留时间、有效区停留距离、有效区停留时间、平台停留距离/总路程比、在平台区的逗留时间/总时间、有效区停留距离/总路程、有效区停留时间/总时间均无明显变化。结论长期食用5年生种植人参原粉、水提物及醇提物对小鼠东莨菪碱致学习记忆获得障碍无明显的改善作用。Aim To o bser ve the long-term effect of Ginseng-5year raw powder、aqueous extract and ethanol extract on learning and memory in mice to provide experimental basis for edible Ginseng. Methods140 Kun Ming mice（70 male and 70 female,18-22g） were divide to 10 groups, normal group,Ginseng raw powder little, middle and large dose group（0.25,0.5 and 1.0g crude drug/ kg）, Ginseng aqueous extract little, middle and large dose group（0.25,0.5 and 1.0g crude drug /kg）,Ginseng ethanol extract little,middle and large dose group（0.25,0.5 and 1.0g crude drug/kg）, 14 mice a group, do the learning and memory abilities experiment of acquisition impairment model mice induced by scopolamine after intragastric administration for 3 months. Result Compared with normal group, incubation period and run-length of Ginseng ethanol extract large dose group had a decreasing trend on the 1st day,but there was no statistical significance, and the run-length shortened on the 4th day（P〈0.05）,at the same time other indicators have no obvious change. There was no obvious change in incubation period, run-length, orientation Angle and swimming speed between Ginseng raw powder little, middle, large dose group, Ginseng aqueous extract little,middle, large dose group, Ginseng ethanol extract little and middle group on the 1st day to 5th day. There was no obvious change in the times of crossing the platform,times of crossing the effective area,distance in the platform,distance in the effective area,standing time in the platform,standing time in the effective area,the ratio of residence time in the platform area/total time,the ratio of residence time in the effective area/total time,the ratio of distance in the effective area /total distance,the ratio of distance in the platform / total distance in 1.5min on the 6th day.Conclusion 5-year-Ginseng raw powder、aqueous extract and ethanol extract have not obvious effect on learning and memory abilities of acquisition impairment model mice induced by scopolamine for edible Ginseng.

关 键 词：五年生种植人参 食用 学习记忆 小鼠 

分 类 号：R285.5[医药卫生—中药学]