静电纺丝聚己内酯纳米纤维支架支持小鼠诱导多能干细胞表达心肌细胞标志物  

Electrospun poly-( ε-caprolactone) nanofibrous scaffolds support cardiomyocyte markers expression of murine induced pluripotent stem cell

作　　者：陈焱[1,2] 何顺舟[3] 李晓莉[1] 曾迪[1] 丁璐[1] 谢江徽[2] 季滨龙 郑强荪[1] 

机构地区：[1]第四军医大学唐都医院心内科,陕西西安710038 [2]解放军第401医院急诊科,山东青岛266071 [3]解放军第401医院心内科,山东青岛266071

出　　处：《基础医学与临床》2016年第4期486-491,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(31271039;31400832)

摘　　要：目的利用多能干细胞与仿生材料支架构建人工心肌,探讨仿生材料支架本身对多能干细胞心肌特异性分化的直接作用,为构建组织工程心肌提供理论支持。方法采用静电纺丝聚己内酯纳米纤维仿生支架,接种小鼠i PSCs(mi PSCs)细胞培养分化15 d,免疫荧光染色与Western blot法检测多能干细胞与心肌细胞特异性标志物。结果mi PSCs特异性表达多能干性标志物Oct4和Nanog,而且能够在聚己内酯纳米纤维支架上集落样增殖、分化;培养15 d后,mi PSCs在支架上分化出心肌特异性标志物c Tn T和MLC2a双重免疫荧光染色阳性的细胞;心肌细胞特异性结构蛋白c Tn T、α-MHC和MLC2的表达水平,支架组全面高于对照组。结论聚己内酯纳米纤维仿生支架支持mi PSCs生长与心肌细胞特异性分化。Objective To explore the possible influence of biomimetic scaffolds on cardiomyocyte（ CM） differentiation of induced pluripotent stem cells（ i PSCs）. Methods The Oct4-GFP＋mouse i PSCs（ mi PSCs） were seeded directly on the poly-（ ε-caprolactone）（ PCL） nanofibrous biomimetic scaffolds,which were fabricated by an electrospun technology,and differentiated for 15 days. The development of CMs was verified by both immunofluorescence staining and Western blot detection. Results The results showed that mi PSCs,expressing Oct4 and Nanog before differentiation,proliferated on the PCL nanofibrous scaffold and grew in colonies during spontaneous differentiation period. Immunofluorescence staining showed the mi PSCs-derived cells were co-labeled with cardiomyocyte specific proteins,such as cardiac Troponin T（ c Tn T） and myosin light chain 2 a（ MLC2 a）. Moreover,Western blot demonstrated that cells derived from mi PSCs after differentiation for 1 5 days expressed higher level of c Tn T,MLC2 and α-myosin heavy chain（ α-MHC） than controls. Conclusions Theses results suggest that the PCL nanofibrous scaffold may support proliferation and cardiomyocyte differentiation of the Oct4-GFP＋mi PSC.

关 键 词：诱导多能干细胞 聚己内酯 纳米纤维支架 心肌细胞 

分 类 号：R318.08[医药卫生—生物医学工程] R542.2[医药卫生—基础医学]