丙烯酰胺对仔鼠大脑额叶皮质神经元DCX和SYN表达的影响  

Effects of acrylamide on expression of doublecortin and synaptophysin in the cerebral frontal cortex of offspring rats

作　　者：李细霞[1] 赵萌萌[1] 张亚琼[1] 杨德慧[1] 李国营[1] 罗利[1] 刘靖[1] 

机构地区：[1]广东药学院基础学院人体解剖学教研室,广州510006

出　　处：《神经解剖学杂志》2016年第2期205-210,共6页Chinese Journal of Neuroanatomy

基　　金：广东省医学科学基金项目(A2012298)

摘　　要：目的:探究丙烯酰胺(acrylamide,ACR)暴露对仔鼠大脑额叶皮质神经元发育的影响。方法:孕鼠随机分为4组,自怀孕第6 d起对照组和实验组分别给予蒸馏水和(50,100,200)μg/ml ACR饮水,直至仔鼠出生21 d。免疫组织化学法观察仔鼠额叶皮质神经元微管相关蛋白(doublecortin,DCX)和突触素(synaptophysin,SYN)的表达情况。结果:ACR实验组和对照组额叶皮质神经元均有DCX和SYN的表达。DCX和SYN免疫阳性产物均为棕黄色颗粒状,表达于胞浆内。与对照组相比,ACR染毒高剂量组(200μg/ml)DCX表达显著减少(P<0.05),而低、中剂量组(50μg/ml和100μg/ml)的减少不明显,无统计学意义(P>0.05);与对照组相比,ACR实验组SYN的表达显著降低,呈剂量依赖性,且组间差异明显(P<0.05)。结论:ACR染毒可能是通过降低DCX和SYN的表达,干扰神经元的迁移分化和突触的形成,从而影响额叶皮质的发育。Objective： To investigate the effect of acrylamide（ACR） on the development of offspring＇s rats frontal cortex neurons. Methods： The pregnant rats were randomizedly divided into 4 groups and provided with doubly evaporated water in control groups and（50,100,200） μg / ml ACR solution via drinking water in experimental groups respectively from gestational day 6 to postnatal day 21. The expression of doublecortin（ DCX） and synaptophysin（ SYN） was observed in the offspring＇s rats frontal cortex neurons by immunohistochemistry. Results： DCX and SYN were expression in both control group and ACR experimental group. DCX and SYN immunoreactive products were brown granular,and locate in cytoplasm. Compared with the control group,the positive expression rate of DCX in the ACR（ 200 μg / ml group） decreased significantly（ P〈 0. 05）,while the decrease of DCX in 50 μg / ml group and 100 μg / ml group had no statistical significance（ P〉 0. 05）. The expression of SYN in the ACR experimental groups were lower than that of the control group in a dose dependent manner,and the difference between groups was significant（ P〈 0. 05）. Conclusion： Exposure to ACR during pregnancy and lactation period may affect the process of neuronal migration,differentiation and synapse formation by decreasing the expression of DCX and SYN,which affects the development of frontal cortex.

关 键 词：丙烯酰胺 神经发育毒性 皮质神经元 微管相关蛋白 突触素 大鼠 

分 类 号：R114[医药卫生—卫生毒理学]