利拉鲁肽在高糖环境下对体外培养的小鼠前成骨细胞MC3T3-E1增殖、分化的影响  

The effects of liraglutide on the proliferation and differentiation of mouse pre-osteoblasts MC3T3-E1

作　　者：李军[1] 靳爽[2] 张娟[1] 张维[3] 周浩峰[4] 张会娟[1] 

机构地区：[1]郑州大学第一附属医院内分泌科,450052 [2]郑州大学第五附属医院 [3]郑州市中心医院 [4]河南科技大学第二附属医院

出　　处：《中华内分泌代谢杂志》2016年第3期232-235,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：河南省二O一三年科技发展计划（编号132300410111）

摘　　要：目的研究不同浓度胰升糖素样肽1（glucagon—likepeptide-1，GLP-1）类似物利拉鲁肽在高糖环境下对小鼠前成骨细胞株MC3T3-E1增殖、分化的影响。方法给予16．5mmoL／L高糖培养基培养小鼠MC3T3-E1细胞，应用不同浓度的利拉鲁肽（10-mol／L、10moL／L、10^-7mol／L）处理培养的成骨细胞48h，同时设立对照组（利拉鲁肽0mol／L），采用CCK-8比色法检测成骨细胞增殖率、实时荧光定量PCR法检测成骨细胞分化标志物-I型胶原（typeIcollagen，COL—I）、碱性磷酸酶（alkalinephosphatase，ALP）和骨桥蛋白（osteopontin，OPN）基因的表达。结果（1）与对照组相比，利拉鲁肽干预组（10^-9moL／L、10^-8mol／L、10^-7molfL）细胞增殖率均显著高于对照组（均P〈0．05），以10^-8mol／L利拉鲁肽促进成骨细胞增殖的作用最显著。（2）与对照组相比，利拉鲁肽干预组COL—I、ALP、OPN的mRNA表达量明显增加（均P〈0．05），在10^-8mol／L浓度组表达量增加最明显。结论在-定浓度范围内利拉鲁肽在高糖环境下能够促进小鼠前成骨细胞MC3T3-E1的增殖和分化。Objective To investigate the effects of liraglutide on the proliferation and differentiation of mouse pre-osteoblasts MC3T3-E1 exposed to higher glucose concentration. Methods MC3T3-E1 cells were cultured and divided into control group and liraglutide group. In liraglutide group, cells were treated with different liraglutide concentrations （10 9 mol/L, 10^-8 mol/L, and 10^-7mol/L, respectively） for 48 hours. Cell proliferation was tested with CCK-8. The mRNA expressions of type I collagen （ COL- I , osteopontin （OPN） , and alkaline phosphase （ALP） were detected using semi-quantitative RT-PCR. Results 1 ） Compared to control group, the proliferation rate of different liraglutide concentration groups （ 10^-9 mol/L, 10-8 mol/L, and 10^-7 mol/L） increased significantly （ all P〈0.05 ）, the proliferation rate was the highest in 10^-8 mol/L liraglutide group. （ 2 ） The expression of COL- I , OPN, and ALP mRNA in liraglutide groups were higher than those in control group （ all P〈 0.05 ）. The optimal concentration of liraglutide was 10 s mol/L. Conclusion Liraglutide within a certain concentration range may improve the proliferation and differentiation of mouse pre-osteoblasts MC3T3-E1.

关 键 词：胰升糖素样肽1类似物 利拉鲁肽 成骨细胞 增殖 分化 

分 类 号：R587.1[医药卫生—内分泌]