植物甾醇诱导人神经母细胞瘤SH-SY5Y细胞凋亡机制初探  

Mechanism of phytosterol induced apoptosis in SH-SY5Y cell

作　　者：刘瑞雪[1] 刘河汝[1] 冯莉[1] 李勇超[1] 张波[1] 

机构地区：[1]北京联合大学应用文理学院,生物活性物质与功能食品北京市重点实验室,北京100191

出　　处：《现代预防医学》2016年第7期1283-1286,共4页Modern Preventive Medicine

基　　金：“十二五”国家科技支撑计划课题(2012BAD33B06)

摘　　要：目的初步探讨植物甾醇诱导人神经母细胞瘤SH-SY5Y细胞凋亡的可能作用机制。方法用不同浓度(0,0.5,2.0,8.0μmol/L)的植物甾醇与SH-SY5Y细胞共同培养,流式细胞术测定细胞周期,Western-blot检测凋亡相关蛋白Caspase-9、Caspase-3的前体及活化体(Pro-Caspase-9、Cleaved-Caspase-9、Pro-Caspase-3、CleavedCaspase-3)的表达。结果不同浓度的植物甾醇可引起SH-SY5Y细胞不同程度的凋亡,并将细胞周期阻滞在G0/G1期,同时引起Caspase相关蛋白的活化。当植物甾醇浓度为2.0μmol/L时,与空白对照组相比,G0/G1期细胞所占百分比从84.35%上升到88.14%,有显著统计学差异(F=25.790,P<0.001);Caspase-3,9有活性剪切体明显增多,与空白对照组相比具有统计学差异(F=30.341,P<0.001,F=18.184,P=0.001)。结论植物甾醇可引起SHSY5Y细胞的凋亡,其机制可能与Caspase-9、Caspase-3的激活有关。Objective To investigate the Mechanism of apoptosis induced by phytosterol in the SH- SY5 Y cell. Methods The human neuroblastoma SH- SY5 Y was treated with phytosterol at different concentration（ 0,0. 5,2. 0,8. 0 μmol / L） in vitro. Cell cycle was evaluated by flow cytometry（ FCM） and apoptosis- related protein was detected by Western- blot. Results Different concentration of plant sterols could induce different degrees of apoptosis of SH- SY5 Y cells and caused cell cycle arrest at G0 /. Apoptosis- related protein（ Caspase- 9 and Caspase- 3） can be activated by phytosterol. Compared with the control group,the percentage of cells at G0 / G1 were greater in group of phytosterol concentration of 2. 0μmol / L（ 84. 35%） than control group（ 88. 14%） with a significant difference（ F = 25. 790,P = 0. 000）,while phytosterol concentration of 2. 0μmol / L had a significant effect on the expression of Cleaved- Caspase- 3（ F = 30. 341,P = 0. 000） and Cleaved- Caspase- 9（ F = 18. 184,P= 0. 001）. Conclusion Apoptosis of SH- SY5 Y cells induced by plant sterols may be associated with the selective activation of Caspase- 9 and Caspase- 3.

关 键 词：植物甾醇 人神经母瘤细胞 细胞凋亡 半胱氨酸-天冬氨酸蛋白酶 

分 类 号：R113[医药卫生—公共卫生与预防医学]