温室培养条件对无花果微枝试管内生根与成活的影响  

Effect of Different Culture Conditions in Green-house on in Vitro Rooting and Survival of Ficus carica Micro-shoots

作　　者：韩会会[1] 符玲巧[1] 柴慈江[1] 苏卫国[1] 

机构地区：[1]天津农学院园艺园林学院,天津300384

出　　处：《天津农学院学报》2016年第1期23-27,共5页Journal of Tianjin Agricultural University

基　　金：国家星火计划项目"优新木本观赏植物组织培养育苗规模化示范"(2008GA610015);天津市星火计划项目"优新木本观赏植物组织培养育苗规模化示范"(08ZHXHNC07000)

摘　　要：为探讨无花果在温室中进行试管内生根的方法与效果,将接种了无花果试管苗茎段的培养瓶分别放在日光温室中的单层遮阳网(透光率约为39.0%)和双层遮阳网(透光率约为16.0%)下,同时以放在恒温室的做对照,进行生根培养,并对生根的试管苗进行气孔观察和移栽试验。结果表明:在温室中培养的无花果试管苗生根率达100%,根长、根数和叶数均显著高于恒温室培养的试管苗,其叶片气孔在未进行开瓶炼苗时已经具有一定的关闭功能。温室中单层网遮阴下培养的无花果试管苗在移栽后不覆膜、不喷雾、空气相对湿度低至60%条件下达到91.2%的移栽成活率。本研究结果可降低无花果试管苗成本,促进其组培快繁技术在生产中的应用。In order to study the method and effect of Ficus carica micro-shoots rooting in vitro in green-house, the cultural bottles containing the sterilized medium supported by soil in which the stem sections of Ficus carica plantlet in vitro were inoculated were respectively located in one shading net（about 39.0% of light transmission rate）or two shading net（about 16.0%of light transmission rate）in green-house for rooting, using the cultural bottles located in the constant temperature room as the control. Then the opening and closing of stoma was observed and the transplanting experiment was done for the Ficus carica plantlet rooted in vitro. The results showed that 100% of the stem sections cultured in green-house rooted and the root length, root number and leaf number of the plantlet cultured in green-house were significantly higher than those of the plantlet cultured in the constant temperature room. Without acclimated by opening bottle the stoma of the plantlet cultured in green-house had closing function in some extent. After transplanted the Ficus carica plantlet had the survival rate of 91.2% under the conditions without plastic film covering, without spraying and the lowest relative air humidity being about 60%. These results can obviously reduce the culture cost of the Ficus carica plantlet in vitro and promote the micro-propagation technology application of Ficus carica to the production.

关 键 词：无花果 微枝 温室 试管内生根 移栽 

分 类 号：S604.3[农业科学—园艺学] S723.123.6