苇蘑液体菌种培养基的优化  

Optimization of Liquid Medium for Weimo Mycelium

作　　者：王翟 赵安[1] 班立桐[1] 黄海东[1] 郭雅聪[1] 杨红澎[1] 王玉[1] 黄亮[1] 

机构地区：[1]天津农学院农学与资源环境学院,天津300384

出　　处：《天津农学院学报》2016年第1期43-45,共3页Journal of Tianjin Agricultural University

基　　金：国家级大学生创新训练计划项目"苇蘑发酵条件优化及多糖口服液的研发"(201510061049);天津市高等学校科技发展基金计划项目"苇蘑液体发酵条件优化及多糖活性的研究"(20140607)

摘　　要：通过比较观察相应的种子液中菌丝球的生长状况及其菌丝球数量、干重等指标,从4种发酵培养基中选出最适合苇蘑菌丝生长的初始发酵培养基,并采用L9(34)正交试验对该培养基进行了优化,确定发酵培养基配方为:葡萄糖15 g/L、乳糖15 g/L、酵母膏4 g/L,自然条件下的p H值。通过最适培养基培养后,苇蘑菌丝干重提高到18.26 g/L。By comparing the mycelium growth, mycelium pellets number and mycelium dry weight of Weimo, the most suitable initial fermentation medium for Weimo was selected from the four types of fermentation culture medium. Then L9（34）orthogonal experiment design was employed to optimize the medium. The determined fermentation medium as the following： glucose 15 g/L, lactose 15 g/L, yeast paste 4 g/L and the nature p H value. The dry mycelium weight of Weimo can reach to 18.26 g/L with the optimum culture medium.

关 键 词：苇蘑 正交试验 菌丝干重 菌丝球 

分 类 号：S646[农业科学—蔬菜学]