南蛇藤素对哮喘小鼠TSLP介导的Th2优势免疫干预的研究  

Study of celastrol on airway inflammation by TSLP in mice with asthma

作　　者：桑琳莉[1] 朱慕云[1] 

机构地区：[1]扬州大学临床医学院苏北人民医院呼吸内科,江苏扬州225001

出　　处：《临床肺科杂志》2016年第5期874-877,共4页Journal of Clinical Pulmonary Medicine

摘　　要：目的观察南蛇藤素对小鼠哮喘模型胸腺基质淋巴生成素(TSLP)介导的气道炎症的影响及机制。方法 24只SPF级BALB/c雌性小鼠随机分成4组。哮喘组、甲强龙组及南蛇藤素组分别予OVA致敏,然后腹腔注射等体积药物(分别为生理盐水、甲强龙及南蛇藤素),再予以雾化吸入1%OVA激发。对照组使用等剂量生理盐水。末次激发24h后,检测各组小鼠血清总Ig E及TSLP含量,支气管肺泡灌洗液(BALF)中IL-4、IL-13水平,HE染色肺组织病理学检测炎症浸润。结果南蛇藤素组小鼠哮喘表现较哮喘组明显改善;其血清总Ig E、TSLP含量及BALF中IL-4、IL-13水平均低于哮喘组(P<0.05);且小鼠肺组织气道炎症明显减轻,但与甲强龙组无异(P>0.05)。结论南蛇藤素组可以通过抑制哮喘小鼠TSLP介导的Th2优势免疫改善其临床症状。Objective To study the influence and mechanism of celastrol on airway inflammation by TSLP in mice with asthma. Methods 24 SPF level BALB/c female mice were randomly divided into four groups. The asthmatic, methylprednisolone and celastrol groups were injected by OVA, then given intraperitoneal injection of equal volume of different drugs （saline, methylprednisolone, and celastrol）. Then mice were given 1% OVA aerosol inhalation while the control group was given only saline. The levels of total IgE and TSLP in serum and IL-4, IL-13 in BALF were measured by ELISA. By I-I＆E staining, inflammation in mice lung tissues were assessed. Results After atomized, the clinical manifestation of the celastrol group improved significantly compared with the asthmatic group mice. The levels of TSLP, total IgE in serum and IL-4, IL-13 in BALF of the celastrol group were less than those in the asthmatic group （P 〈 0.05 ）, and there was no significant difference in the levels of those factors between the celastrol group and the methylprednisolone group （P 〉 0. 05 ）. In addition, the inflammatory cell infiltrating in lung tissue of mice in the celastrol group decreased significantly compared with the asthmatic group, but there was no difference in airway inflammation between the celastrol group and the methylprednisolone group. Conclusion Celastrol maybe have an effect treatment on asthma through inhibiting Th2 advantage immune mediated by TSLP in asthmatic mice.

关 键 词：南蛇藤素 TSLP Th2优势免疫 哮喘 

分 类 号：R562.25[医药卫生—呼吸系统]