木榄提取物及单体化合物对SH-SY5Y神经细胞抗氧化研究  

Antioxidation of Bruguiera Gymnorrhiza Extracts and Its Compounds on SH-SY5Y Cells

作　　者：周媛[1,2] 薛艳红[2] 刘朝霞[2] 

机构地区：[1]西安外事学院医学院,西安710077 [2]天然产物研究与利用湖北省重点实验室(三峡大学),湖北宜昌443002

出　　处：《三峡大学学报（自然科学版）》2016年第2期88-91,共4页Journal of China Three Gorges University：Natural Sciences

基　　金：国家自然科学基金项目(31540065);天然产物研究与利用湖北省重点实验室开放基金(2015NP03);三峡大学人才启动基金项目(KJ2009B001)

摘　　要：研究了中国海南红树植物木榄提取物及其单体化合物对SH-SY5Y神经细胞的抗氧化作用.对木榄成分提取分离、结构鉴定后,用H2O2处理人神经瘤母细胞（SH-SY5Y细胞）,建立细胞损伤模型,以MTT法检测木榄提取物及单体化合物的细胞增殖作用与抗氧化活性.结果表明木榄乙醇提取物、石油醚萃取部位、乙酸乙酯萃取部位及部分单体化合物能很好地对抗H2O2诱导的SHSY5Y细胞损伤.单体化合物在低剂量（1.25~5μg·mL-1）时量效关系良好,随着浓度的增加,对SH-SY5Y细胞的抗氧化作用逐渐增强.木榄能能很好地对抗H2O2诱导的SH-SY5Y细胞损伤,对SH-SY5Y细胞具有显著的抗氧化作用,该作用与减少胞内氧自由基有关,其机制有待进一步研究.To investigate antioxidation of the Chinese Hainan mangrove plant,Bruguiera gymnorrhiza,the compounds were isolated and purified with silica column chromatography and sephadex LH-20 column chromatography and HPLC methods.Their structures were identified by physicochemical analysis,spectroscopic analysis（1 H-NMR,13C-NMR and MS）.After extraction and purification,H2O2 oxidative damage model to human neuroblastoma strains SH-SY5 Ycells was established;and then the cell toxicity and the antioxidation under oxidative stress were evaluated by MTT method.The ethanol extract,petroleum ether extract and ethyl acetate extract of B.gymnorrhiza showed better antioxidant activities for SH-SY5 Ycells.Among the compounds oleic acid（BGP-1）,physcion（BGP-3）,Mansonone H（BGP-4）,Catacerebroside B（BGP-5）,caffeic acid（BGE-9）and rhein（BGE-10）had more significant protective effects and showed the dose-dependent effect at the low concentrations（1.25-5μg/mL）.It is concluded that the B.gymnorrhizacan be developed as a potential drug for neurodegenerative diseases.

关 键 词：木榄 SH-SY5Y细胞 H2O2 抗氧化 

分 类 号：Q421[生物学—神经生物学]