荧光定量PCR检测淋球菌、沙眼衣原体和解脲脲原体结果分析  

Detection and analysis of NG,CT and UU by fluorescence quantitative PCR

作　　者：张欠欠[1] 仵恒立 胡军婷 成俊珍[2] 任勇[2] 杨晗[1] 马莹[1] 

机构地区：[1]延安大学医学院,延安716000 [2]延安大学附属医院,延安716000

出　　处：《中国人兽共患病学报》2016年第3期312-314,共3页Chinese Journal of Zoonoses

基　　金：陕西省教育厅科研项目(No.14JK1836);延安大学青年专项基金资助项目(No.YDQ2013-03);陕西省大学生创新创业训练计划项目(No.1415)~~

摘　　要：目的了解延安市皮肤性病科门诊患者泌尿生殖道淋球菌（NG）、沙眼衣原体（CT）及解脲脲原体（UU）的感染现状及其特点,为临床提供依据。方法采用实时荧光聚合酶链式反应技术（FQ-PCR）对784例患者的泌尿生殖道分泌物的NG、CT和UU核酸进行检测。结果 784例患者中,NG、CT和UU的阳性检出率分别为15.43%、34.69%和30.87%,女性UU感染率明显高于男性（P〈0.01）,男性CT感染率明显高于女性（P〈0.05）,NG感染率男性虽高于女性,但无性别差异（P〉0.05）。混合感染检出率为19.64%,其中CT＋UU双重感染的阳性检出率最高,为10.20%。不同年龄感染情况显示,感染率以21~40岁年龄段最高。结论泌尿生殖道感染患者NG、CT和UU阳性检出率不尽相同,阳性患者大多年龄在青壮年时期,应作为性传播疾病防治工作中的重点对象。混合感染较为常见,临床应进行3种病原体的检测。We investigated the prevalence of Neisseria gonorrhoeae（NG）,Chlamydozoa trachomatis（CT）and Ureaplasma urealyticum（UU）infections in Yan′an,China.The genital tract infections in 784 patients were deteeted by using FQ-PCR for providing clinical treatment evidence of NG,CT and UU.The positive rates of NG,CT and UU were 15.43%,34.69%and 30.87%,respectively.The incidence of Ureaplasma urealyticum（UU）was significantly higher in male group than in female group（P〈0.01）,while was lower in case of Chlamydozoa trachomatis（CT）infection（P〈0.05）.Mixed infection was19.64%,CT and UU mixed infection was dominant in urogenital tract infection（UTI）patients（10.20%）.This study also showed that infection rate in age group 21-40 years was higher.There was significant difference in positive rate of NG,CT and UU in Urogenital tract infection（UTI）patients.The most affected age group was young adults,indicating significant sexual transmitted diseases.Mixed infection was common,and more attention should be paid to these three kinds of pathogenic diseases detection clinically.

关 键 词：淋球菌 沙眼衣原体 解脲脲原体 实时荧光聚合酶链式反应 

分 类 号：R374[医药卫生—病原生物学]