云南一种新型蝙蝠博卡病毒的鉴定与分析  

Detection and identification of a new bat bocavirus species in Yunnan

作　　者：李兴宇[1] 陈刚[2] 夏乐乐[1] 周卫国[2] 徐琳[1] 余静[2] 邱薇[2] 张富强[2] 范泉水[2] 涂长春[1] 胡挺松[2] 何彪[1] 

机构地区：[1]军事医学科学院军事兽医研究所,吉林省人兽共患病防控重点实验室,长春130122 [2]成都军区疾病预防控制中心,昆明650118

出　　处：《军事医学》2016年第3期207-212,233,共7页Military Medical Sciences

基　　金：国家自然科学基金-云南省联合基金资助项目(U1036601);国家自然科学基金资助项目(31572529)

摘　　要：目的进一步发现云南新型蝙蝠病毒,并鉴定云南省蝙蝠携带博卡病毒(bocavirus)的遗传多样性。方法对采自景洪市的26只三叶蹄蝠进行了病毒宏基因组学分析,根据其结果对博卡病毒进行了PCR检测和全基因分析。结果与结论根据检测结果,从3只(11.5%)蝙蝠的肠道中发现了一种新型博卡病毒;通过全基因组扩增和分析,发现该病毒全长5203 nt,编码NS1、NP和VP1/VP2蛋白,分析显示该病毒NS1和VP1基因分别与博卡病毒代表种犬博卡病毒(canine bocavirus)1和犬微小病毒(canine minute virus)有最高的氨基酸序列相似性,达到58.7%和53.3%。依据国际病毒分类委员会病毒新种判断标准,NS1蛋白基因氨基酸序列相似性低于85%,为博卡细小病毒属新种,因而该病毒是一新博卡病毒种;该研究为进一步了解我国蝙蝠携带病毒的多样性提供了参考,同时为研究博卡病毒的宿主范围和进化关系提供了重要的基础数据。Objective To discover more novel bat viruses and molecularly characterize bat-borne bocavirus diversity in Yunnan. Methods Twenty-six Aselliscus stoliczkanus were sampled in Jinghong, Yunnan, and subjected to viral metagenomic analysis. Specific PCR was used to detect any bocavirus in these samples based on the metagenomic result,while full genome was amplified and compared with other bocaviruses. Results and Conclusion Totally,3 of the 26（ 11. 5%） bats were positive for bocavirus,the full genome of which contained 5203 nucleotides and could encode NS1,NP and VP1 / VP2 proteins. Phylogenetic analysis showed that this virus shared up to 58. 7% and 53. 3% amino acid identities with canine bocavirus 1 and canine minute virus. According to ICTV criteria（ 85% amino acid sequence identity）on a new species of bocavirus,this virus could be a novel species within genus Bocaparvovirus. This study provides important data to better understand viral diversity in bats and to uncover the relationship between bocavirus and its hosts.

关 键 词：云南 蝙蝠 博卡病毒 进化分析 

分 类 号：S852.65[农业科学—基础兽医学]