基于实时定量PCR技术的秀丽隐杆线虫肠道食物菌鉴定方法  

Quantitative analysis of intestinal bacteria in Caenorhabditis elegans using qPCR

作　　者：于周龙 高艳[2] 张成岗[1,2] 

机构地区：[1]广西医科大学,南宁530021 [2]军事医学科学院放射与辐射医学研究所,蛋白质组学国家重点实验室,全军军事认知与心理卫生研究中心,北京100850

出　　处：《军事医学》2016年第3期248-251,共4页Military Medical Sciences

基　　金：国家重点基础研究发展计划资助项目(2012CB518200);国家自然科学基金资助项目(81371232;81100979;81070741)

摘　　要：目的建立基于实时定量PCR技术的秀丽隐杆线虫肠道食物菌鉴定方法。方法采用实时定量PCR(real-time quantitative polymerase chain reaction,qP CR)分别对1、20、50只线虫进行肠道食物菌检测,分析成虫期和衰老期线虫肠道食物菌的变化情况。结果 qP CR可检测到单只线虫肠道食物菌含量,可准确反映不同样本之间肠道食物菌含量的变化。结论基于qP CR技术的线虫肠道食物菌鉴定方法可对线虫肠道食物菌进行准确定量,具有成本低、灵敏度高、特异性好的优势。Objective To establish a method for detecting intestinal bacteria in Caenorhabditis elegans. Methods The gut flora number of 1,20 or,50 C. elegans was quantified and compared using real-time quantitative PCR（ qP CR）.Results The gut flora of a single C. elegans could be detected using qP CR method,which could also reflect the difference in the number of gut bacteria between different samples. Conclusion The qP CR method can be uased to accurately quantify intestinal bacteria even in only one C. elegans and has the advantages of low-cost,high-sensitivity and good-specificity.

关 键 词：秀丽隐杆线虫 实时定量聚合酶链反应 肠道食物菌 

分 类 号：R440[医药卫生—诊断学]