分光光度法测定红树秋茄中总黄酮含量  

Spectrophotometric Determination of Total Flavonoids in Kandelia candel

作　　者：计燕萍[1] 唐岚[2] 

机构地区：[1]嘉兴学院,嘉兴314001 [2]浙江工业大学,杭州310014

出　　处：《北方药学》2016年第4期5-6,共2页Journal of North Pharmacy

摘　　要：目的：建立红树秋茄中总黄酮含量的测定方法。方法：采用紫外分光光度法,通过单因素试验优选显色方法和显色条件,建立总黄酮含量的测定方法。结果：采用Na NO2-Al（NO3）3-Na OH法,以芦丁作为对照品,在0.02~0.1mg/m L浓度范围内线性关系良好,r2=0.9999,平均回收率为103.02%（RSD=2.2%.n=8）。结论：该方法准确、灵敏、重现性好,可用于秋茄总黄酮含量的测定。Objective：To establish a method for determination of the content of total flavonoids in Kandelia candel.Method：single factor tests were adopted to optimize colorimetry methods and conditions by UV FDS,determination of the content of total flavonoids was established. Result： Selecting rutin as a reference substance,Na NO2-Al（NO3）3-Na OH method was adopted,rutin had a liner relationship with absorbance in the range of 0.02 ~0.1mg/m L,r2 =0.9999,average recovery was 103.02% with RDS of 2.2%,n =8.Conclusion：Na NO2-Al（NO3）3-Na OH method appeared to be an accurate,reliable and repeatable system fordetermination of total flavonoids in Kandelia candel.

关 键 词：秋茄总黄酮 紫外分光光度法 含量测定 

分 类 号：R284.1[医药卫生—中药学]