PRRSV特异性肽-N对CSFV弱毒疫苗免疫猪骨髓细胞因子分泌的影响  

The effect of PRRSV specific peptide-N on the secretion of cytokines in CSFV immuned swines' bone marrow

作　　者：孔惟博 孙宏伟[1] 梁志选[2] 任少敏[1] 刘芳宁[1] 张玉珠[1] 徐鹏[1] 舒馨[1] 赵建增 高英杰[1] 

机构地区：[1]吉林大学动物医学学院,吉林长春130062 [2]天津市动物疫病预防控制中心,天津300402 [3]华威特(北京)生物科技有限公司,北京100085

出　　处：《中国兽医学报》2016年第4期549-552,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30972193)

摘　　要：本试验探讨PRRSV特异性肽-N对CSFV弱毒疫苗免疫猪骨髓细胞因子分泌量的影响。选择已免疫CSFV弱毒疫苗的猪为实验动物,提取四肢长骨骨髓,进行T淋巴细胞的分离培养。分别在培养后的24、72 h,用PRRSV特异性肽N1、N2对所培养细胞进行刺激,再进行培养12 h,经离心方法得到细胞上清液,最终检测上清液中细胞因子IFN-γ、IL-12、IL-10的分泌含量变化。结果显示,PRRSV特异性肽-N对于免疫猪骨髓中IFN-γ的分泌影响较小,能够抑制IL-12的分泌,不利于机体免疫反应,对IL-10的分泌影响复杂,需进一步研究。This paper intends to discuss the effect of specific peptide-N of PRRSV on the bone mar- row cytokines＇ secretion in swines. Swines immunized with attenuated vaccine of CSFV were se- lected as the experimental animals. The bone marrow was operated out of bodies antiseptically in vivo. Then T lymphoeytes in the bone marrow were isolated and cultured by our own method. At 24 and 72 h after culture,the culturing cells were stimulated by both of specific peptides N1 and N2. After another 12 h,the supernatant liquid after centrifugation was used to test the secretion of IFN-γ,IL-12 and IL-10. The results showed that the peptide-N displayed a little effect on the se- cretion of IFN-γ in immunized swines＇ bone marrow,however it could inhibit the secretion of IL- 12,which is not conducive to the immune response. The complex effect of peptide-N on the secre- tion of IL-10 still need to be researched further. Meanwhile, the experimental data could provide more useful resources for the vaccine and precaution of PRRSV.

关 键 词：PRRSV特异性肽 骨髓 IFN-γ IL-12 IL-10 

分 类 号：S852.65[农业科学—基础兽医学]