慢性丙型肝炎患者外周血RIG-Ⅰ基因表达与丙型肝炎病毒载量及Ⅰ型干扰素基因的相关性  

Expression of RIG-Ⅰgene in peripheral blood of patients with chronic hepatitis C and its ;correlations with hepatitis C virus load and typeⅠinterferon gene expression

作　　者：王方平[1] 张平安[1] 牛志立[1] 

机构地区：[1]武汉大学人民医院检验科,430060

出　　处：《中华临床感染病杂志》2016年第1期19-23,共5页Chinese Journal of Clinical Infectious Diseases

摘　　要：目的：研究慢性丙型肝炎（ CHC）患者外周血单个核细胞（ PBMC）中维甲酸诱导基因Ⅰ（RIG-Ⅰ）mRNA表达量及其与丙型肝炎病毒（HCV）载量及Ⅰ型干扰素（IFN）基因之间的关系。方法选择2014年7月至2015年7月武汉大学人民医院收治初入院未治疗的101例CHC患者，并选择同期的健康体检者69名作为对照。采用实时荧光定量聚合酶链反应检测RIG-Ⅰ、IFNα、IFNβmRNA表达和HCV载量，并采用Pearson法分析RIG-ⅠmRNA表达量与IFNα和IFNβmRNA表达量以及HCV载量的相关性。结果设健康对照组mRNA表达量为1，CHC组RIG-Ⅰ、IFNα和IFNβ基因mRNA表达量分别为0．082、0．022和0．156，两组比较差异具有统计学意义（ t＝7．83、3．65和2.13，P值均＜0．05）；HCV RNA载量＜（1＆#215;106） IU／mL 组RIG-Ⅰ基因表达量为HCV RNA载量≥（1＆#215;106） IU／mL组的1．79倍，差异具有统计学意义（t＝2.60， P＜0．05）；CHC患者RIG-ⅠmRNA表达量与IFNα、IFNβmRNA表达量呈正相关（r＝0．247和0．489， P＜0．05），与HCV载量无相关性（r＝0．187， P＞0．05）。结论与健康人群相比，CHC患者RIG-ⅠmRNA表达量降低，且与IFNα和IFNβmRNA表达量呈正相关。Objective To detect the expression of retinoic acid-inducible gene Ⅰ （ RIG-Ⅰ） in peripheral blood mononuclear cells （PBMCs） of patients with chronic hepatitis C （CHC）, and to explore its correlations with type Ⅰinterferon gene expression and serum level of hepatitis C virus .Methods A total of 101 na？ve patients with CHC were enrolled from Renmin Hospital of Wuhan University during July 2014 and July 2015, and 69 healthy subjects served as controls .The expressions of RIG-Ⅰ, IFNαand IFNβmRNA in PBMCs and serum level of HCV RNA were detected by real-time quantitative polymerase chain reaction.Pearson correlation analysis was performed to investigate the correlations of RIG-Ⅰ mRNA expression with IFNα/βmRNA expression and serum level of HCV RNA .Results The relative expressions of RIG-Ⅰ, IFNα, IFNβmRNA to healthy controls in CHC group were 0.082, 0.022 and 0.156, respectively （t=7.83, 3.65 and 2.13, all P〈0.05）.RIG-Ⅰ mRNA expression in patients with HCV loads 〈（1 ＆#215;106 ） IU/mL was 1.79 times higher than that in patients with HCV loads≥（1 ＆#215;106 ） IU/mL （t=2.60, P 〈0.05）.Pearson correlation analysis showed that the expression of RIG-Ⅰ mRNA was positively correlated with that of IFNαmRNA and IFNβmRNA （r=0.247 and 0.489, P〈0.05）, but not correlated with HCV load （r=0.187, P〉0.05）.Conclusion Expression of RIG-ⅠmRNA decreases in patients with CHC , and is positively correlated with that of IFNαand IFNβmRNA.

关 键 词：肝炎 丙型 慢性 维甲酸诱导基因Ⅰ 干扰素Ⅰ型 聚合酶链反应 

分 类 号：R51[医药卫生—内科学]