胡颓子叶中总黄酮及三种指标成分的含量测定方法研究  

Content Determination Method of Total Flavonoids and 3 Maker Compounds in Elaeagnus pungens

作　　者：覃勤[1] 钟大妹 吕倩[1] 徐海燕[1] 葛月宾[1] 

机构地区：[1]中南民族大学药学院,湖北武汉430074

出　　处：《时珍国医国药》2016年第3期535-538,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(No.81470181);国家自然科学基金青年项目(No.31000156);湖北省自然科学基金群体项目(No.2013CFA013)

摘　　要：目的建立测定胡颓子叶中总黄酮及槲皮素、山柰酚和异鼠李素含量的方法。方法采用紫外分光光度法,以芦丁为对照品,以三氯化铝（Al Cl3）显色,在411 nm处测定胡颓子叶中总黄酮的含量。采用水解-HPLC法同时测定胡颓子叶中槲皮素、山柰酚和异鼠李素的含量。色谱条件采用Ultimate KAQ-C18柱（250 mm×4.6 mm,5μm）,流动相为甲醇-0.1%磷酸梯度洗脱,流速为1.0 ml/min,检测波长为360 nm,柱温为30℃。结果芦丁的质量浓度在0.0056~0.028mg/ml与吸光度呈良好线性关系（r=0.9997）,平均加样回收率为100.99%,RSD=1.89%（n=9）。槲皮素、山柰酚、异鼠李素3种黄酮苷元质量浓度分别在9.91~59.46μg/ml（r=0.9999）、46.6~372.8μg/ml（r=0.9998）、0.99~19.8μg/ml（r=0.9998）与峰面积积分值呈良好的线性关系,平均加样回收率分别为101.87%,101.21%,97.62%（RSD均小于2%,n=6）。胡颓子叶样品中总黄酮、槲皮素、山柰酚和异鼠李素的含量分别为11.94,0.34,1.89,0.03 mg/g。结论该方法简便可靠,重复性好,可作为胡颓子叶中黄酮类物质的含量测定方法。Objective To establish the method for the content determination of total flavonoids and quercetin,kanpferol and isorhamnetin in Elaeagnus pungens. Methods Using rutin as control,the content of total flavonoids in Elaeagnus pungens was determined by UV spectrometry. The contents of quercetin,kaempferol and isorhamnetin were determined by HPLC after hydrolysis.The analysis was carried out on an UltimateAQ-C18column（ 250 mm × 4. 6 mm,5 μm） and detected with an UV detector at the wavelength of 360 nm. Gradient elution was carried out with methanol-0. 1% phosphoric acid at the flow rate of 1. 0 m L /min. The column temperature was set at 30 ℃. Results The linear range was 0. 0056 ~ 0. 028 mg / m L for rutin（ r = 0. 9997）with an average recovery of 100. 99%（ RSD = 1. 89%,n = 9）. The linear range of quercetin,kaempferol and isorhamnetin was9. 91 ~ 59. 46 μg / ml（ r = 0. 9999）,46. 6 ~ 372. 80 μg / ml（ r = 0. 9998） and 0. 99 ~ 19. 8 μg / m L（ r = 0. 9998）,respectively.The average recoveries of the three components were 101. 87 %,101. 21 % and 97. 62 %,respectively. The total flavonoids and3 maker compounds in Elaeagnus pungens were 11. 94,0. 34,1. 89,0. 03 mg / g,respectively. Conclusion The method is simple,reliable and repeatable,and can be used for the simultaneous determination of flavonoids in Elaeagnus pungens.

关 键 词：胡颓子叶 总黄酮 槲皮素 山柰酚 异鼠李素 含量测定 紫外分光光度法 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]