三种黄酮类化合物抗肿瘤活性比较  

Anti-tumor Effect of Three Kinds of Flavonoids

作　　者：王月华[1] 李爱峰[2] 张丽[1] 付崇罗[1] 赵洁[1] 玄红专[1] 

机构地区：[1]聊城大学生命科学学院,山东聊城252059 [2]聊城大学化学与化工学院,山东聊城252059

出　　处：《时珍国医国药》2016年第3期543-546,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.31201860);山东省自然科学基金(No.ZR2012CQ003;No.ZR2014BQ023)

摘　　要：目的研究金丝桃苷、白麻苷和次野鸢尾黄素三种黄酮类化合物对不同肿瘤细胞和血管内皮细胞的细胞毒性。方法正常培养的乳腺癌细胞(MCF-7,MDA-MB-231)、肺腺癌细胞(A549)及血管内皮细胞(HUVECs)经不同浓度的金丝桃苷、白麻苷和次野鸢尾黄素(20,40,80,160μmol·L^(-1))分别处理24 h和48 h,倒置显微镜观察细胞形态,SRB法检测细胞存活率。结果金丝桃苷和白麻苷对三种肿瘤细胞均没有显著的细胞毒性,但抑制去除血清条件下HUVECs细胞凋亡;次野鸢尾黄素对乳腺癌MDA-MB-231细胞抑制较强,对乳腺癌MCF-7和肺癌A549细胞抑制较弱。结论金丝桃苷对内皮细胞具有保护功效,而次野鸢尾黄素是一种潜在的抗肿瘤黄酮类化合物。Objective To study the antitumor activities of hyperoside, quercetin-3-O-sophorosid and irisflorentin.Methods The normal cultured human lung carcinoma（ A549）,human breast cancer（ MCF-7,MDA-MB-231） and human umbilical vein endothelial cells（ HUVECs） were treated with different concentrations of hyperoside,quercetin-3-O-sophorosid and irisflorentin（ 20,40,80,160 μmol·L^（-1）） for 24 and 48 h,respectively. The cell viability was measured by SRB method. Results Hyperoside and quercetin-3-O-sophoroside had no significant cytotoxicity on three tumor cells,however,the two compounds can protect HUVECs deprived of serum. The cytotoxicity of irisflorentin on MCF-7 and A549 cells was much lower than on MDA-MB-231 cells and induced HUVECs deprived of serum apoptosis. Conclusion Hyperoside can protect HUVECs,whereas irisflorentin is a potential antitumor agent.

关 键 词：金丝桃苷 白麻苷 次野鸢尾黄素 抗肿瘤 

分 类 号：R284.1[医药卫生—中药学]