柱前衍生化-HPLC法测定乙醇消毒液中乙醇的含量  

Pre-column derivatization-HPLC method for analyzing the content of ethanol in ethanol disinfection solution

作　　者：范继青[1] 陈念祖[1] 黄滔敏[1] 

机构地区：[1]复旦大学附属眼耳鼻喉科医院药剂科,上海200031

出　　处：《中国临床药学杂志》2016年第2期89-92,共4页Chinese Journal of Clinical Pharmacy

基　　金：上海市科委基金项目(编号11DZ1972100)

摘　　要：目的建立一种测定乙醇消毒液中乙醇含量的HPLC法。方法以甘油为内标，乙醇和甘油经对甲苯磺酰异氰酸酯（TSIC）衍生化后衍生化产物用HPLC法测定。色谱柱为Agilent C18（150mm×4．6mm，5μm），流动相为乙腈-磷酸缓冲液（0．01mol·L^-1 KH2P04，磷酸调pH至2．5）（35：65），检测波长227nm，流速1mL·min^-1，柱温25℃。结果在该条件下，乙醇在质量浓度2．5—200mg·L^-1内线性良好（r〉0．9995，n=7）。回收率为97％～103％（n=5），RSD〈3％（n=5）。衍生化产物在12h内稳定。测得样品中乙醇的标示量百分含量为90％～110％。结论经验证，该方法快速、简便、结果准确、重复性好，适合于乙醇消毒液中乙醇的含量测定。AIM To develop an HPLC method to analyze the content of ethanol in ethanol disinfection solution. METHODS Ethanol and glycerol （internal standard, IS） were derivatized with p-toluenesulfonyl isocyanate （TSIE） and analyzed by HPLC. The derivatized IS and derivatized ethanol were separated on an Agilent C18（ 150 mm × 4.6 mm,5 μm） column, using a UV detector and the wavelength was 227 nm. The mobile phase was CH3CN- 0.01 mol·L-1KH2PO4 （adjusted to pH 2.5 with phosphoric acid）（35：65）. The temperature of the column was 25 ℃ and the flow rate of the mobile phase was 1 mL· min- 1. RESULTS Ethanol had good linearity （ r 〉 0. 999 5, n = 7） in the range of 2.5 - 200 mg·L-1. The recovery values were between 97% and 103%, RSD 〈 3.0% （n = 5）. The samples were stable in 12 h and qualified between 90% and 110% . CONCLUSION The method is proved to be simple, fast, accurate, reli- able and suitable for the quality control of ethanol in ethanol disinfection solution.

关 键 词：高效液相色谱法 柱前衍生化 乙醇消毒液 甘油 甲苯磺酰异氰酸酯 

分 类 号：R927.2[医药卫生—药学] O657.72[理学—分析化学]