黄连素对实验性糖尿病大鼠肝糖原的影响  

Effect of Berberine on Hepatic Glycogen of Experimental Diabetic Rats

作　　者：徐行仙[1] 何新康[2] 

机构地区：[1]浙江省江山市人民医院,浙江江山324100 [2]浙江医学高等专科学校,浙江杭州310053

出　　处：《中国中医药科技》2016年第2期161-162,174,共3页Chinese Journal of Traditional Medical Science and Technology

摘　　要：目的:观察黄连素对实验性糖尿病大鼠肝糖原的影响,探讨黄连素的降血糖作用机制。方法:采用链脲佐菌素尾静脉注射复制实验性糖尿病模型,实验分为正常组、模型组、黄连素低剂量组、黄连素高剂量组,每组10只。分组给药4周后,测定大鼠的空腹血糖、肝糖原含量、糖异生转化率、肝脏葡萄糖激酶(GK),观察肝糖原PAS染色结果。结果:黄连素明显降低实验性糖尿病大鼠空腹血糖,抑制糖异生,降低糖异生转化率,增强GK活性,增加肝糖原含量,增强肝糖原PAS染色。结论:黄连素对实验性糖尿病大鼠肝糖原的影响可能通过抑制糖异生,增强GK活性来实现的。Objective： To investigate the mechanism of Berberine on lowering blood sugar by observing the effect of Berberine on hepatic glycogen of diabetes rats. Methods： Diabetic models were made by injecting STZ, experiment was divided into 4 groups ,normal ,model, berberine high ,low dose groups, 10 rats in each group,they were given medicine or NS for 4 weeks. The fasting plasma glucose, glycogen, gluconeogenesis conversion rate, liver glucokinase （GK）activity, PAS staining of glycogen were datermined. Results： Berberine could signifi- cantly reduce fasting glucose, inhibite gluconeogenesis, reduce gluconeogenesis conversion rate, and enhance GK activity, increase glycogen content and enhance glycogen PAS stain in diabetic rats. Conclusion：Effects of Barberine on glycogen of experimental diabetic rats may be related to inhibiting gluconeogenesis and enhance GK activity.

关 键 词：糖尿病 实验性 黄连素 肝糖原 糖异生 葡萄糖激酶(GK) 大鼠 

分 类 号：R285.5[医药卫生—中药学]