36份苜蓿种质材料遗传多样性的SSR分子标记  

Genetic Diversity Analysis of 36 Alfalfa Accessions Based on SSR Markers

作　　者：王娟[1,2] 杜建材[2] 王照兰[2] 刘佳月[2] 李青丰[1] 

机构地区：[1]内蒙古农业大学生态环境学院,内蒙古呼和浩特010018 [2]中国农业科学院草原研究所,内蒙古呼和浩特010010

出　　处：《中国草地学报》2016年第2期20-25,33,共7页Chinese Journal of Grassland

基　　金：中国农业科学院创新工程草种质资源与育种团队;国家科技支撑计划课题(2011BAD17B01)

摘　　要：采用SSR分子标记方法,对36份不同苜蓿种质材料的基因组DNA进行扩增,并用6%的变性聚丙烯酰胺凝胶电泳对扩增结果进行了检测,结果表明：9对SSR引物共扩增出146个等位基因位点,扩增出的DNA片段大小在100-2000bp之间,其中多态位点72个,多态位点百分率43.57%;基因多样性指数平均值为0.1502,Shannon信息指数平均值为0.2047;多态信息量（PIC）平均值为0.8962。用Structure2.3软件对36份苜蓿材料进行结构分析,可将其划分为1个混合群体和4个特定类群,供试材料具有显著异质性,可用于苜蓿育种及优良基因挖掘利用。The genome DNAs of 36 alfalfa accessions were amplified with the method of SSR molecular markers.The results showed that 146 fragments were amplified by nine SSR primer combinations and the DNA fragments length was between 100-2000bp which include 72 polymorphic loci,and the percentage of polymorphic loci was 43.57%.The average genetic index,Shannon information index and polymorphism information content（PIC）was 0.1502,0.2047 and 0.8962,respectively.The Strunture2.3software was applied to analysis the structure of 36 alfalfa accessions,which can be divided into one mixed group and four specific groups.Because of the significant heterogeneity,the alfalfa materials can be used in breeding and exploring special genes.

关 键 词：苜蓿 SSR标记 遗传多样性 

分 类 号：S541[农业科学—作物学]