石斛合剂等降糖中药方对衰老糖尿病大鼠胰腺PCNA、NF-κBp65的影响  

Effects of Shihu Compound with Blood Glucose Decline Chinese Medicine on PCNA and NF-κBp65 Expressions in Pancreas of Aged Diabetes Mellitus Model Rats

作　　者：王晓宁[1] 郑燕芳[1] 余文珍[1] 高尤亮[2] 杨秀珍[3] 林雅[1] 张捷平[1] 刘如玉[1] 何才姑[1] 李长征[1] 郑良朴[1] 施红[1] 

机构地区：[1]福建中医药大学,福州350122 [2]福建中医药大学附属第二人民医院,福州350003 [3]江苏省泰州市人民医院,泰州225300

出　　处：《世界中医药》2016年第2期296-300,共5页World Chinese Medicine

基　　金：福建中医药大学校管科研课题(编号:X2012017);福建中医药大学重点学科专项校管课题(编号:X2014024-学科);福建省中青年教师教育科研项目(一般项目A类科技项目)(编号:JA15240);福建省自然科学基金项目(编号:2014J01354)

摘　　要：目的：比较石斛合剂等降糖中药方对衰老糖尿病模型大鼠胰岛细胞增殖凋亡相关因子PCNA、NF-κBp65的影响,以遴选出保护胰岛细胞功能、防治老年糖尿病的方药。方法：采用Wistar雌性大鼠制备衰老DM模型大鼠,随机分为：衰老对照组、衰老DM模型组、衰老DM石斛合剂组、衰老DM生脉散组,并给予药物治疗4周后,检测血清FBG、GSP等浓度;免疫组化法检测胰腺组织中PCNA和NF-κBp65的表达。结果：与衰老DM模型组相比：石斛合剂组大鼠FBG与GSP显著降低（P〈0.05~0.01）,生脉散组亦降低;且治疗组之间差异有统计学意义（P〈0.01）;石斛合剂组胰腺PCNA表达非常显著（P〈0.01）,生脉散组表达亦增加（P〈0.05）,2个治疗组之间比较差异有统计学意义（P〈0.01）;但治疗组该2项指标均未达到衰老对照组水平。各组胰岛内NF-κBp65表达由多到少顺序依次如下：石斛合剂组〉衰老DM模型组〉生脉散组〉衰老对照组。结论：与生脉散相比,石斛合剂能够更明显地改善衰老DM大鼠血糖;二者均能促使DM大鼠胰腺组织PCNA表达,提示其可促使胰腺细胞增殖,改善被破坏的内环境稳态,呈现石斛合剂〉生脉散的趋势。各组胰岛内NF-κBp65表达由多到少顺序依次如下：石斛合剂组〉衰老DM模型组〉生脉散组〉衰老对照组。推测：患DM后,机体产生应激反应,石斛合剂可以促使NF-κB参与应激反应,调动了机体潜力;生脉散则减弱NF-κB参与应激反应。在防治老年糖尿病方面,石斛合剂较生脉散更具优势。Objective： To observe the effect of Shihu Compound（ DC） and other Chinese medicine on declining blood glucose and their effects on PCNA and NF-κBp65 of aged diabetes Mellitus model rats. Methods： The aged diabetic model rats were divided into control group,model group,DC group and PRPB（ Shengmai San） group. They were given drugs for four weeks. The concentrations of FBG and GSP in sernm were measured,and the PCNA and NF-κBp65 in pancreas of aged diabetic rats were assayed by immunohistochemistry. Results： Compared with the model group,the level of FBG and GSP decreased in DC group and PRPB group（ P 〈 0. 01）. And PCNA expression was evidently heightened in the two treatment group,and there were significant differences between the two groups（ P 〈 0. 01）. The level of FBG and GSP and the PCNA expression has not reach the level of that of the aged control group. The NF-κBp65 expression in pancreas was DC treated group 〉 aged DM model group 〉 PRPB treated group〉 aged control group. Conclusion： DC could more significantly improve the blood glucose and enhance PCNA expression in model group than PRPB group. DC could better improve the internal environment homeostasis in aged diabetic rats via promoting the pancreatic cell proliferation than PRPB. It could be deduced that DC enables NF-κB to involve in stress response in DM and mobilize the body potential,but PRPB functions the contrary. DC has better effect in treating aged DM than PRPB.

关 键 词：衰老糖尿病模型 胰腺 PCNA NF-ΚBP65 石斛合剂 

分 类 号：R692.3[医药卫生—泌尿科学]