TGF-β1、MMP-9在哮喘大鼠气道重建模型中的表达及丹参的干预作用  

Expression of TGF-β1、MMP-9 in airway remodeling in Asthma Rats Model and Effects of Salvia Miltiorrhiza

作　　者：陈静[1] 赵文娟[1] 李玉卿[1] 夏永辉[1] 袁嘉丽[1] 

机构地区：[1]云南中医学院,昆明650500

出　　处：《世界中医药》2016年第3期479-482,共4页World Chinese Medicine

基　　金：云南省教育厅重大专项项目(编号:ZD2013010);云南省教育厅科学研究基金项目(编号:2013Y241);云南省高校中医药调节机体微环境应用基础研究科技创新团队项目

摘　　要：目的:观察TGF-β1、MMP-9在哮喘大鼠气道重建模型中的动态变化,探讨丹参干预气道重建的作用机制。方法:SPF级SD成年雄性大鼠70只随机分为正常对照组、哮喘模型组(哮喘2周组、哮喘4周组、哮喘8周组)、丹参干预组(丹参2周组、丹参4周组、丹参8周组)3个大组,7个小组。用卵蛋白(OVA)-氢氧化铝混悬液致敏与激发建立大鼠哮喘气道重塑模型,激发2、4、8周后处死。观察哮喘大鼠肺组织病理变化,采用ELISA法检测大鼠肺组织TGF-β1、MMP-9水平。结果:哮喘大鼠气道炎性细胞浸润及支气管平滑肌增厚,随着激发时间的延长逐渐加重;同时,哮喘大鼠肺组织TGF-β1、MMP-9水平逐渐升高,哮喘4周组、哮喘8周组较正常对照组显著升高(P<0.05、P<0.001);使用丹参干预后,丹参8周组较哮喘8周组显著降低(P<0.001)。结论:气道重建的程度随着哮喘发作及时间推移而发生动态变化;TGF-β1、MMP-9参与哮喘气道重建过程;活血化瘀药丹参可通过抑制TGF-β1、MMP-9水平而干预阻断哮喘气道重建。Objective： To observe the dynamic changes of TGF-β1 and MMP-9 in the airway remodeling of asthma rat model and explore the mechanism of salvia miltiorrhiza（ Danshen） in intervening airway remodeling. Methods： Seventy healthy adult male rats were randomly divided into the normal control group,asthma model group（ subdivided into two-week asthmatic group,fourweek asthmatic group and eight-week asthmatic group） and salvia miltiorrhiza group（ subdivided into two-week salvia miltiorrhiza group,four-week salvia miltiorrhiza group,and eight-week salvia miltiorrhiza group） with a total of seven groups with 10 rats in each. Asthmatic airway remodeling rats model was established with ovalbumin and Al（ OH） 3,and rats were observed 2,4 and 8weeks after induced model respectively. The pathological changes of the lung tissue were observed. The level of TGF-β1 and MMP-9 in lung tissue was detected by ELISA. Results： Airway inflammatory cells infiltrated in airway tissue and the thickness of bronchial smooth muscle increased. With the prolongation of the stimulating time,TGF-β1 and MMP-9 level increased gradually in the asthma model group. Compared with the normal control group,there were significant differences（ P〈0. 05,P〈0. 001） in the four-week asthmatic group and eight-week asthmatic group. After salvia miltiorrhiza was applied,the TGF-β1 and MMP-9 level decreased. Compared with the eight-week asthmatic group,there were significant differences（ P〈0. 001） in the eight-week salvia miltiorrhiza group. Conclusion： The changes of airway remodeling in asthma are dynamic process and TGF-β1and MMP-9 may intervene in the changes of airway remodeling process. Salvia miltiorrhiza improve the state of airway remodeling in asthmatic rats by inhibiting the level of TGF-β1 and MMP-9.

关 键 词：哮喘 气道重建 TGF-Β1 MMP-9 丹参 

分 类 号：R285.5[医药卫生—中药学]