回神颗粒减轻中度创伤性脑损伤大鼠脑内炎性反应的机制  

The Mechanism of Huishen Granule Alleviating Intracerebral Inflammatory Response on Moderate Traumatic Brain Rats

作　　者：崔俊波[1,2] 陈宝贵[2] 

机构地区：[1]中国中医科学院博士后科研流动站,北京100700 [2]天津中医药大学附属武清中医院,天津301700

出　　处：《世界中医药》2016年第3期507-509,共3页World Chinese Medicine

基　　金：全国名老中医传承工作室建设项目[编号:国中医药人教发(2011)41];全国中医药传承博士后资助项目[编号:国中医药人教函(2013)240号];天津市"131"创新型人才第一层次人选培养工程[编号:津人才(2013)9]

摘　　要：目的:探讨回神颗粒减轻中度创伤性脑损伤(Traumatic Brain Injury,TBI)后脑内炎性反应的作用环节。方法:应用液压性脑损伤装置建立中度TBI模型,创伤压力为(170±10)k Pa,作用时间为(20±2)ms,随机将大鼠分为6组:正常组、假手术组、模型3 d组、模型7 d组、治疗3 d组、治疗7 d组。治疗3 d组和治疗7 d组于创伤后即刻用2 m L药液(含回神颗粒0.27 g)灌胃1次,此后再分别于每日早晚各灌胃1次,每次2 m L药液。采用RT-PCR检测6组动物损伤区脑组织内ICAM-1的变化。结果:与正常组比较:模型3 d组、模型7 d组、治疗3 d组ICAM-1的表达明显升高,具有统计学意义(P<0.01);治疗7 d组ICAM-1的表达的升高无统计学意义(P>0.05)。治疗3 d组ICAM-1的表达较模型3 d组明显降低,差异具有统计学意义(P<0.05);治疗7 d组ICAM-1的表达与模型7 d组比较,差异具有统计学意义(P<0.05)。结论:回神颗粒通过从转录水平抑制ICAM-1的表达,进而减轻脑内炎性反应,避免TBI后脑损伤的进一步加重。Objective： This research aims to investigate the mechanism of Huishen Granule alleviating intracerebral inflammatory response in rats with traumatic injury injury（ TBI）. Methods： Using the equipment of fluid percussion injury（ FPI） to establish moderate FPI model in SD rats. The traumatic pressure was（ 170 ± 10） k Pa,traumatic time was（ 20 ± 2） ms. Randomly divided rats into 6 groups： normal group,sham group,model 3 day group,model 7 day group,experimental 3 day group,experimental 7day group. Experimental 3 day group and experimental 7 day group were given 2 m L fluid（ contain 0. 27 g Huishen Granule） into rats＇ stomach instantly after trauma,and then given 2 m L fluid in the morning and at night. Using real-time polymerization chain reaction（ RT-PCR） to examine the change of ICAM-1 in all 6 groups. Results： Compared with normal group,the ICAM-1 of model3 day group,model 7 day group and experimental 3 day group increased obviously（ P〈0. 01）. However,compared with model 3day group,the ICAM-1 of experimental 3 day group descended significantly（ P〈0. 05）. Compared with model 7 day group,the ICAM-1 of experimental 7 day group declined obviously（ P〈0. 05）. Conclusion： The mechanism of Huishen Granule alleviating intracerebral inflammatory response was through prohibiting the expression of ICAM-1 and avoid aggravation of TBI.

关 键 词：创伤性脑损伤 继发性脑损伤 脑内炎性反应 中性粒细胞 细胞间黏附分子-1 回神颗粒 

分 类 号：R285.5[医药卫生—中药学]