基于HPCE指纹图谱的土鳖虫质量控制研究  

Research of Quality Control of Ground Beetle by HPCE Fingerprint

作　　者：张月娥 陈伟韬[2] 罗文汇[2] 陈昭[2] 

机构地区：[1]广州番禺区中医院,广州510000 [2]广东省第二中医院(广东省中医药工程技术研究院),广州510095

出　　处：《世界中医药》2016年第3期533-535,共3页World Chinese Medicine

基　　金：广东省中医药局建设中医药强省科研课题(编号:20141027)

摘　　要：目的:在建立基于高效毛细管电泳指纹图谱的基础上,建立更为全面、准确的土鳖虫药材质量控制与评价方法;方法:采用毛细管电泳法(HPCE法),使用熔融硅胶毛细管(75μm i.d.,总长30 cm,有效长度21 cm),以硼砂为缓冲液(10mmol/L,p H 9.7),进样电压5 kv 10 s,分离电压15 kv,检测波长214 nm,建立不同批次、来源土鳖虫药材的指纹图谱。根据所得指纹图谱,找出其中的共有峰,并使用峰面积归一化法计算各组分含量;结果:土鳖虫药材中存在7个共有峰,含量测定结果显示,上述成分的含量在不同批次、来源药材中存在统计学意义;结论:该法快速、高效、准确度高,精密度好,能够更为全面、系统地评价土鳖虫药材的质量。Objective： To establish a comprehensive and precise method for quality control of Ground Beetle by the High Performance Capillary Electrophoresis（ HPCE）; Methods： A fused silica capillary（ 75 μm i. d.,21 cm effective length,30 cm total length） was used as column,borate solution was used as buffer solution（ 10 mmol / L,p H 9. 7）,inject voltage was 5 kv 10 s,separation voltage was 15 kv and detection wavelength was 214 nm,common peaks were extracted from fingerprints of different batches and origins of Ground Beetle,and content of each compound was calculated by area normalization method. Results： Total seven peaks from the fingerprint were identified,and as the content determination result indicated,content of each compound had significant differences. Conclusion： HPCE is fast,efficient,accurate and precise,which can be used as a comprehensive and systematical method for quality control of Ground Beetle.

关 键 词：土鳖虫 HPCE 指纹图谱 质量控制 

分 类 号：R282[医药卫生—中药学]