基于铅离子-苯基荧光酮-TritonX-100体系的氡荧光猝灭检测新方法  

A new method for the fluorescence quenching detection of radon based on the lead ion-phenylfluorone-TritonX-100 system

作　　者：龙敏芝[1] 沈燚[1] 邓涵[1] 田刚[1] 宋春丽[1] 吕昌银[1] 

机构地区：[1]南华大学公共卫生学院卫生检验系,湖南衡阳421001

出　　处：《中国卫生检验杂志》2016年第7期913-916,931,共5页Chinese Journal of Health Laboratory Technology

基　　金：国家自然科学基金(81473021)

摘　　要：目的研究铅离子对苯基荧光酮-Triton X-100体系的荧光猝灭特性,建立基于子体铅的氡无辐射危害荧光猝灭检测新方法。方法用0.2%HAc溶液采集氡辐射的子体铅。在Tris-HAc缓冲介质中,Pb^(2+)与PF形成配位物,经非离子表面活性剂Triton X-100敏化,体系发生灵敏的荧光猝灭效应,ΔF与Pb^(2+)浓度呈良好的线性关系,与氡的辐射强度之间具有良好的数学定量关系。据此,建立氡的荧光猝灭检测新方法。结果 Pb^(2+)浓度为1.44 ng/ml^300 ng/ml时,铅离子-苯基荧光酮-Triton X-100体系ΔF与ρ之间呈现良好的线性关系,标准曲线的回归方程为ΔF=8.78ρ+82.02,相关系数(r)=0.999 1,检出限为0.43 ng/ml。氡辐射强度[D,×10~3(Bq·h)/m^3]为8.76、17.90、56.49、66.38时,氡样品测定溶液的ΔF值与D值之间呈现良好的线性关系,回归方程为ΔF=10.37D+365.25,r=0.990 6。结论本方法灵敏、准确,操作简单,在无辐射危害的条件下可检测放射性气体氡。Objective To find out the fluorescence quenching properties of lead ion- phenylfluorone- Triton X- 100 system,and to establish a new method for the detection of the fluorescence quenching of the radon ion radiation hazard. Methods Lead progeny in radon radiation was collected with 0. 2% HAc solution. In Tris- HAc buffer medium,Pb（2＋）and PF formed coordination compound through the non ionic surface active agent Triton X- 100 sensitization,the sensitive fluorescence quenching phenomenon appeared. The fluorescence quenching degree（ ΔF） showed a good linear relationship with Pb（2＋）. The radiation intensity of radon had a good mathematic quantitative relationship. According to the above,a new method of fluorescence quenching detection of radon was established. Results When the concentration of Pb（2＋）was 1. 44 ng / ml- 300 ng / ml,ΔF of the lead ion- phenylfluorone- Triton X- 100 system and ρ had a good linear relationship,the regression equation of the standard curve was ΔF = 8. 78ρ ＋ 82. 02 and correlation coefficient was 0. 999 1,the detection limit was 0. 43 ng / ml. When radon radiation strength[D,× 10-3（ Bq·h） /m3]were 8. 76,17. 90,56. 49,66. 38,ΔF value of radon determination solution showed a good linear relationship with D value and ΔF = 10. 37 D ＋ 365. 25 and the correlation coefficient was 0. 990 6. Conclusion The method was sensitive,accurate and simple,and it was a simple method to detect radioactive gas radon in the condition of no radiation hazard.

关 键 词：氡 铅 苯基荧光酮 荧光猝灭 TRITONX-100 

分 类 号：O657.31[理学—分析化学]