Force degradation behavior of glucocorticoid deflazacort by UPLC：isolation,identification and characterization of degradant by FTIR,NMR and mass analysis  

作　　者：Rajesh Deshmukh Lata Sharma Muktika Tekade Prashant Kesharwani Piyush Trivedi Rakesh K.Tekade 

机构地区：[1]Macleods Pharmaceuticals Ltd., Kachigam, Daman, 396320, India [2]School of Pharmaceutical Science, Rajiv Gandhi Prodyogiki Vishwavidyalaya, Gandhi Nagar, Bhopal 462036, India [3]TIT College of Pharmacy, Technocrats Institute of Technology, Anand Nagar, Bhopal 462021, India [4]Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA [5]The University of Texas Southwestern Medical Center, Advanced Imaging Research Center, Dallas, TX 75390, USA [6]The International Medical University, School of Pharmacy, Department of Pharmaceutical Technology, Kuala Lumpur,57000, Malaysia

出　　处：《The Journal of Biomedical Research》2016年第2期149-161,共13页生物医学研究杂志（英文版）

摘　　要：In this investigation,sensitive and reproducible methods are described for quantitative determination of deflazacort in the presence of its degradation product.The method was based on high performance liquid chromatography of the drug from its degradation product on reverse phase using Acquity UPLC BEH C18columns（1.7 urn,2.1 mm ×150 mm） using acetonitrile and water（40：60 V/V） at a flow rate of 0.2 mL/minute in UPLC.UV detection was performed at 240.1 nm.Deflazacort was subjected to oxidative,acid,base,hydrolytic,thermal and photolytic degradation.The drug was found to be stable in water and thermal stress,as well as under neutral stress conditions.However,forced-degradation study performed on deflazacort showed that the drug degraded under alkaline,acid and photolytic stress.The degradation products were well resolved from the main peak,which proved the stability-indicating power of the method.The developed method was validated as per ICH guidelines with respect to accuracy,linearity,limit of detection,limit of quantification,accuracy,precision and robustness,selectivity and specificity.Apart from the aforementioned,the results of the present study also emphasize the importance of isolation characterization and identification of degradant.Hence,an attempt was made to identify the degradants in deflazacort.One of the degradation products of deflazacort was isolated and identified by the FTIR,NMR and LC-MS study.In this investigation,sensitive and reproducible methods are described for quantitative determination of deflazacort in the presence of its degradation product.The method was based on high performance liquid chromatography of the drug from its degradation product on reverse phase using Acquity UPLC BEH C18columns（1.7 urn,2.1 mm ×150 mm） using acetonitrile and water（40：60 V/V） at a flow rate of 0.2 mL/minute in UPLC.UV detection was performed at 240.1 nm.Deflazacort was subjected to oxidative,acid,base,hydrolytic,thermal and photolytic degradation.The drug was found to be stable in water and thermal stress,as well as under neutral stress conditions.However,forced-degradation study performed on deflazacort showed that the drug degraded under alkaline,acid and photolytic stress.The degradation products were well resolved from the main peak,which proved the stability-indicating power of the method.The developed method was validated as per ICH guidelines with respect to accuracy,linearity,limit of detection,limit of quantification,accuracy,precision and robustness,selectivity and specificity.Apart from the aforementioned,the results of the present study also emphasize the importance of isolation characterization and identification of degradant.Hence,an attempt was made to identify the degradants in deflazacort.One of the degradation products of deflazacort was isolated and identified by the FTIR,NMR and LC-MS study.

关 键 词：Deflazacort forced degradation degradant characterization 

分 类 号：R927[医药卫生—药学]