DryLab软件辅助建立同时测定6种细胞色素P450酶探针底物的HPLC法  

Computerized optimization of high-performance liquid chromatographic method by using DryLab to test and isolate six cytochrome P450 probe substrates

作　　者：杨文宁[1] 潘艳丽[3] 杨瑞瑞[1] 马晓云[1] 陈新晶[1] 罗志强[1] 王豪[1] 刘洋[2] 

机构地区：[1]北京中医药大学,100102 [2]北京中医药大学中药学院,100102 [3]中国中医科学院中医药信息研究所办公室,北京100700

出　　处：《国际中医中药杂志》2016年第4期350-354,共5页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金（81274042）;北京中医药大学在读研究生项目（2015-JYB-XS090）

摘　　要：目的 采用DryLab软件辅助建立可同时测定6种细胞色素P450酶（cytochrome P450, CYP450）探针底物的HPLC法。方法 选择6种CYP450酶探针底物为研究对象，应用DryLab软件辅助建立合适的液相色谱分离方法，并进行方法学考察。结果 借助DryLab软件辅助建立的色谱方法可较好的将6种探针底物分离，分离度均大于2.00，各探针底物在一定范围内峰面积与浓度呈线性关系，R＞0.999 8，回收率在86.38%～110.29%范围内，其RSD在1.69%～3.80%之间，日内和日间精密度分别在0.42%～2.01%和1.36%～2.29%范围内。结论 通过DryLab软件建立的色谱方法可同时检测6种探针底物，缩短了色谱方法的开发周期。Objective DryLab software was used to assist high performance liquid chromatography （HPLC） method to test and isolate six Cytochrome P450 （CYP450） probe substrates. Methods Six CYP450 probe substrates were selected and the right HPLC method was developed and validated with the assistance of DryLab software. Results The new HPLC method with the assistance of DryLab software could test and isolate six probe substrates with degrees of isolation more than 2.00. The correlation coefficients （R〉 0.999 8） indicated high linear correlation between the concentrations and the peak areas among six probe substrates. Recovery studies showed good results for all the probe substrat from 86.38% to 110.29%. And therelative standard deviation （RSD） ranged from 1.69% to 3.80% with its intra-day and inter-day precision ranging from 0.42% to 2.01%, and 1.36% to 2.29%, respectively. Conclusions The developed HPLC method with the assistance of DryLab could test and isolate six probe substrates with shortertime than the HPLC method alone.

关 键 词：细胞色素P450酶系统 高效液相色谱 DryLab软件 探针底物 

分 类 号：R96[医药卫生—药理学] O657.72[医药卫生—药学]