百令胶囊对大鼠肝移植后肝肾功能影响的实验研究  

Experimental Study of the Effect of Bailing Capsule on liver and kidney function of Rats After Liver Transplantation

作　　者：李龙珠[1] 刘家军[2] 郭俐宏[3] 李莉[3] 

机构地区：[1]湖北省十堰市中医医院妇产科,十堰442012 [2]湖北省十堰市中医医院消化科,十堰442012 [3]湖北医药学院机能实验室,十堰442000

出　　处：《世界中医药》2016年第1期127-130,共4页World Chinese Medicine

摘　　要：目的:观察百令胶囊对大鼠50%同种异体肝脏移植术后肝肾功能的影响,探讨百令胶囊对肝移植大鼠肝、肾缺血/再灌注损伤的保护机制。方法:将10只大鼠设为空白对照组,另40只大鼠随机分为模型组、百令胶囊组,采用双袖套法对2组大鼠进行同种异体交叉肝脏移植术,于百令胶囊灌胃治疗14 d后测定肝功能谷草转氨酶(AST)、谷丙转氨酶(ALT)及胆红素(TBIL);肾功能中胱抑素C(Cys C)、尿素氮(BUN)及血清肌酐(Cr);血清丙二醛(MDA)、乳酸脱氢酶(LDH)及血清内源性超氧化物歧化酶(SOD);并籍此评价百令胶囊对大鼠同种异体肝脏移植术后肝肾功能的影响。结果:百令胶囊组AST、ALT、TBIL、LDH、Cys C、BUN、Cr明显降低,血清SOD含量升高,与模型组及本组治疗前比较,P<0.05,差异均有统计学意义。结论:百令胶囊可提高SOD以清除肝脏移植术自由基的产生,抑制脂质过氧化反应,减轻排异反应及肝、肾缺血/再灌注对肝肾功能的损害,促进肝肾功能恢复,对移植肝脏有一定的保护作用。Objective： To observe the effect of Bailing Capsule on function of liver and kidney of rat after allograft liver transplantation and to explore the protective mechanism of Bailing capsule of liver,renal ischemia / reperfusion injury in liver transplantation.Methods： We randomly divided ten rats into blank control group,40 model group and Bailing capsule group,and perform allograft liver transplantation cross on two groups of rats by two cuff method. After taking Baling capsule for 14 days,the aspartate aminotransferase（ AST）,alanine aminotransferase（ ALT）,total bilirubin（ TBIL）,cystatin C in renal function（ Cys C）,blood urea nitrogen（ BUN）,serum creatinine（ Cr）,serum malondialdehyde（ MDA）,lactate dehydrogenase（ LDH） and serum superoxide dismutase（ SOD） level were detected to evaluate the effect of Bailing capsule on function of liver and kidney of the rats. Results： The AST,ALT,TBIL,LDH,Cys C,BUN and Cr of Bailing capsule group decreased,serum SOD elevated significantly compared with the model group and the situation before treatment. P〈0. 05,the difference was significant. Conclusion： Bailing capsule can promote the function of SOD in removing the free radical operation after liver transplantation,restrain the inhibiting lipid peroxidation,reduce the damage of rejection reaction and hepatic and renal ischemia / reperfusion,improve the recovery of liver and kidney,which all shows that it has protective effect during the process of liver transplantation.

关 键 词：大鼠 百令胶囊 肝脏移植 肝肾功能 

分 类 号：R285.5[医药卫生—中药学]