胰岛素样生长因子-1抗体对注射A型肉毒毒素后运动终板功能恢复的影响  

Effect of insulin-like growth factor 1 antibody on motor endplate function after botulinum toxininjection

作　　者：潘丽珍[1] 靳令经[1] 潘有贵[1] 郑玉果[2] 管强[1] 郭艳[1] 聂志余[1] 

机构地区：[1]同济大学附属同济医院神经内科,上海200065 [2]中国生物技术发展中心,北京100036

出　　处：《中华神经医学杂志》2016年第4期345-349,共5页Chinese Journal of Neuromedicine

基　　金：（1）国家自然科学基金（81000481）（2）上海市青年科技启明星计划（10QA1407500）（3）上海市卫生局科研课题计划（2009Y070）

摘　　要：目的探讨胰岛素样生长因子-1抗体（IGF-1Ab）对大鼠腓肠肌肌注A型肉毒毒素（Btx-A）后运动终板功能的影响。方法90只SD大鼠按随机数字表法分为4组，第1组为对照组，第2组为Btx-A组，第3、4组为抗体组（Ab2μg、Ab20μg组）。第2、3、4组于大鼠右侧腓肠肌局部注射Btx—A0．5μ（0．1mL），第3天时第3、4组分别在同一位点注射IGF—lAb2μg、20μg（0．1mL），动态测量大鼠腓肠肌肌力及乙酰胆碱酯酶染色阳性区平均吸光度值变化。结果注射Btx-A后，Btx-A组大鼠腓肠肌肌力由注射前12．34±0．16g降至7．70±0．90g，其他各组肌力亦较注射前减退；第14、28、42、56、70天，IGF—1Ab组（第3、4组）肌力较Btx-A组均减小，差异有统计学意义（JD〈0．05），且第42～70天第4组肌力低于第3组，差异有统计学意义（P〈0．051；注射部位运动终板染色平均吸光度值演变呈相同趋势。结论IGF-1Ab可抑制Btx-A肌注后大鼠腓肠肌运动终板功能的恢复。Objective To investigate the effect of insulin-like growth factor 1 antibody （IGF-1Ab） on motor endplate fi.mction after injection ofbotulinum toxin A （Btx-A）. Methods The total 90 male SD rats were randomly divided into 4 groups： control group, Btx-A group and 2 ug and 20 ug IGF-IAb groups. In Btx-A and IGF-1Ab groups, a volume of 0.5 U （0.1 mL） Btx-A was intramuscularly injected into a site in the right gastrocnemius muscle; on day 3, equal volumes （0.1 mL） of IGF-1Ab with a dosage of 2 ug and 20 ug were injected to the 2 ug and 20 μg IGF-1Ab groups respectively at the same site. The gastrocnemius muscle strength and the mean optical density （MOI） value of the positive reaction zone of acetylcholine esterase staining were evaluated at different time points. Results The gastrocnemius muscle strength increased from 12.34±0.16 g before injection to 7.70±0.90 g after injection in the Btx-A group; the gastrocnemius muscle strength decreased in other groups after injection of Btx-A; on day 14, 28, 42, 56 and 70, the muscle strength oflGF-1Ab groups was significantly lower than that of Btx-A group （P〈0.05）, and on day 42-70, the value of muscle strength of 20 μg IGF-1Ab group was signficantly lower than that of 2 μg IGF-1Ab group （P〈0.05）. The MOI valuesof the positive reaction zone changed with the same trend. Conclusion IGF-1Ab can suppress the restore of motor endplate function after injection of Btx-A.

关 键 词：肉毒毒素 胰岛素样生长因子-1抗体 肌力 

分 类 号：R965[医药卫生—药理学]