基于双光子诱导荧光的肿瘤标志物CA125的实时动态可视化活检  

Real Time Dynamic Visualization Biopsy of Tumor Marker CA125 Based on Two-photon Induced Fluorescence

作　　者：黄池宝[1,2] 曾伯平[1] 陈华仕[1] 陈晓远[2] 

机构地区：[1]遵义师范学院化学化工学院,贵州遵义563002 [2]韶关学院农业科学与工程学院,广东韶关512005

出　　处：《分析测试学报》2016年第4期492-496,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金(21562050);贵州省第八批科技创新人才团队建设专项基金(黔科合人才团队(2015)4007号);贵州省科学技术基金项目(黔科合J字[2015]2146号);贵州省教育厅自然科学重点研究项目(黔教合KY字[2014]296);贵州省教育厅省级本科教学工程建设项目(黔教[2014]JXGChcb);遵义市"15851人才精英工程"项目((2015)4007);遵义市红花岗区科学技术项目(遵红科合社字[2015]18)

摘　　要：糖链抗原CA125(Ag)是肿瘤早期及癌变期最重要的标识物,该文利用抗CA125单克隆抗体Ab125(Ab)特异性亲合抗原的原理,用制备的双光子荧光标记探针LP标记抗体(LP-Ab)以特异性识别CA125,用近红外激光激发抗原-抗体复合物(LP-Ab·Ag),利用复合物荧光实现对肿瘤标志物CA125的量化检测和实时动态成像。该方法简单易行,克服了单光子荧光检测中的光致毒与光漂白,且能显著提高成像的分辨率、清晰度与灵敏度,对肿瘤及癌变的早期诊断与预防具有重要意义。Carbohydrate antigens CA125（Ag） are the most important markers in early stage of tumor and cancer stage.Antibodies Ab125（Ab） to carbohydrate antigens were labeled to form probe-labeled antibodies LP-Ab using the prepared two-photon fluorescence probe LP.On the basis of the antigen-antibody specificity affinity principle,LP-Ab could combine with carbohydrate antigen into a complex LP-Ab·Ag which emitted strong two-photon fluorescence to accomplish the high sensitive biopsy and the real-time dynamic high-resolution imaging of the tumor markers.The easy,simple and quick method could not only overcome photodamage,photobleaching and tissue auto-fluorescence disturbance,but also be used to observe and study the dynamic transfer mechanisms of the tumor markers in cells and the mechanism of carcinogenesis.The research in this project is very useful in the early diagnosis and prevention of tumor and canceration.

关 键 词：糖链抗原CA125 Ab125 免疫荧光标记 双光子 活检 

分 类 号：O657.3[理学—分析化学] S852.43[理学—化学]