棉花耐盐相关基因GhVP的表达及功能分析  

Expression and Functional Analysis of a Salt Tolerance-Related Gene from Cotton

作　　者：赵小洁[1,2] 穆敏[1,2] 陆许可[1] 阴祖军[1] 王德龙[1] 王俊娟[1] 樊伟丽[1] 王帅[1] 叶武威[1] 

机构地区：[1]中国农业科学院棉花研究所/棉花生物学国家重点实验室/农业部棉花遗传改良重点开放实验室,河南安阳455000 [2]河南大学,河南开封475000

出　　处：《棉花学报》2016年第2期122-128,共7页Cotton Science

基　　金：国家科技重大专项--转基因生物新品种培育(2014ZX0800504B)

摘　　要：为了研究Gh VP基因在棉花中的表达特性和功能,克隆了棉花Gh VP基因,构建p BI121-Gh VP∷GFP荧光表达载体,采用基因枪技术转化洋葱内表皮细胞,结果显示Gh VP基因编码蛋白定位于细胞质膜上。将构建的p BI121-Gh VP∷GFP荧光表达载体转化棉花花粉,花粉荧光明显增强,说明该基因可以在棉花中表达,为Gh VP基因可以在棉花中表达提供了依据,为培育棉花耐盐新材料奠定了基础。To develop an expression system to study the function of the Gh VP gene in cotton（Gossypium hirsutum）, we successfully cloned Gh VP and used it to construct a p BI121-Gh VP∷GFP fluorescence expression vector. We transformed the vector into onion（Allium cepa） epidermal cells using gene gun technology and found that the Gh VP protein was located on the cytomembrane. The p BI121-Gh VP∷GFP vector was then transformed into cotton pollen. Detection of green fluorescence in the pollen indicated that the gene was successfully expressed from the p BI121-Gh VP∷GFP vector. These results lay the foundation for the study of Gh VP in cotton and the development of salt tolerance breeding.

关 键 词：棉花 GH VP 亚细胞定位 花粉 瞬时表达 

分 类 号：S562[农业科学—作物学]