胆管癌中垂体肿瘤转化基因和碱性成纤维细胞生长因子的表达及意义  

Expression of pituitary tumor transforming gene and basic fibroblast growth factor and its relationship with clinical pathological characteristics of bile duct carcinoma

作　　者：余健雄[1] 陈国容[2] 

机构地区：[1]广东省开平市中心医院普通外科,529300 [2]福建医科大学附属第一医院神经外科,福州350000

出　　处：《广东医学》2016年第5期678-681,共4页Guangdong Medical Journal

基　　金：广东省医学科研基金立项课题(编号:A2012254)

摘　　要：目的探讨垂体肿瘤转化基因(PTTG)和碱性成纤维细胞生长因子(b-FGF)与胆管癌发生发展的关系,进而为胆管癌的诊断以及早期治疗奠定坚实的基础。方法选择58例胆管癌组织和20例正常胆管组织(取自胆结石临床患者),应用免疫组织化学方法对这两种组织PTTG以及b-FGF的表达水平进行检测。结果胆管癌组织的PTTG以及b-FGF的阳性表达率分别为81.03%、74.14%,高于正常胆管组织(P<0.05)。PTTG与b-FGF在胆管癌组织中的表达呈正相关。结论 PTTG在胆管癌组织中的高表达诱导了多肽生长因子bFGF的细胞外分泌与高表达,b-FGF又诱导PTTG的表达,两者的高表达与胆管癌的生物学行为密切相关;PTTG与b-FGF在胆管癌组织中的表达呈正相关,两者联合检测对胆管癌的恶性程度、治疗及预后判断具有指导意义。Objective To investigate human pituitary tumor transforming gene（ PTTG） and basic fibroblast growth factor（ b- FGF） expression in cholangiocarcinoma and their correlation,as well as relevant clinicopathological parameters,for diagnosis and early treatment of cholangiocarcinoma. Methods 58 cases of cholangiocarcinoma tissue were collected as the observation group,and 20 cases of normal bile duct tissue from patients with gallstones were collected as the control group. The expression of PTTG and b- FGF in the two groups was detected by immunohistochemistry,and the results were studied and analyzed. Results The positive rate of PTTG and b- FGF was 81. 03% and 74. 14%,respectively. Positive expression rates in cholangiocarcinoma,were significantly higher than in the control group（ P〈0. 05）.PTTG expression in cholangiocarcinoma was positively correlated with the expression of b- FGF. Conclusion High expression of PTTG in cholangiocarcinoma induces extracellular secretion and high expression of b- FGF. Meanwhile,B-FGF also induces the expression of PTTG. High expression of PTTG and b- FGF is closely related to the biological behaviors of cholangiocarcinoma. Expressions of PTTG and b- FGF in cholangiocarcinoma are positively correlated. Combined detection of PTTG and b- FGF can help determine the degree of malignancy,guide treatment and predict prognosis of cholangiocarcinoma.

关 键 词：胆管癌 垂体肿瘤转化基因 碱性成纤维细胞生长因子 免疫组织化学 

分 类 号：R644[医药卫生—外科学]