竹荪加工中废弃物多糖水解液的胶束毛细管电泳研究  

Polysaccharide Hydrolysate in Dictyophora Processing Waste by Micellar Electrokinetic Chromatography

作　　者：白新伟[1] 王毅红[1] 陈定梅[1] 田玲[1] 

机构地区：[1]六盘水师范学院化学与化学工程系,贵州六盘水553004

出　　处：《分析科学学报》2016年第2期265-268,共4页Journal of Analytical Science

基　　金：贵州省市校科技合作(联合基金)项目(黔科合LH字[2015]7624号)

摘　　要：本文建立了胶束毛细管电泳测定竹荪加工废弃物的多糖组成的新方法。考察了缓冲溶液、表面活性剂、运行电压、温度等因素对分离效果的影响。选择25mmol/L硼酸盐(pH=9.4)-20mmol/L十二烷基磺酸钠(SDS)为电泳介质,分离电压为15kV,分离温度为25℃,9min中内可实现对竹荪加工中废弃物中多糖的单糖组分的分离检测。A highly efficient method for the determination of monosaccharides in dictyophora processing waste by using micellar electrokinetic chromatography was developed.The optimum conditions were as follows voltage of 15 kV,methanol medium containing 25 mmol/L borate-20 mmol/L sodium dodecyl sulfonate（SDS）.Under the optimum conditions,monosaccharides could be separated within 9 min.The detection limits（at a signal-to-noise ratio of 3）are in the range of 0.81-1.60mmol/L with linear ranges from 5to 100 mmol/L.The developed method was applied to the determination of monosaccharides in dictyophora processing waste with recoveries ranging from 94.3%-103.4%.

关 键 词：竹荪废弃物 多糖 胶束毛细管电泳 十二烷基磺酸钠 

分 类 号：O657.8[理学—分析化学]