斜纹夜蛾半人工饲料的改进  

Improvement of semi-artificial diet for Spodoptera litura

作　　者：张南[1] 陈巨龙 仵均祥[1] 

机构地区：[1]西北农林科技大学旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [2]陕西省烟草研究所,陕西西安710061

出　　处：《西北农林科技大学学报（自然科学版）》2016年第4期109-113,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：中国烟草总公司项目"烟草有害生物调查研究"(KJ-2010-04)

摘　　要：【目的】改进斜纹夜蛾半人工饲料配方,为斜纹夜蛾室内批量饲养奠定基础。【方法】设计4种半人工饲料配方,于温度(27±0.5)℃、相对湿度(70±5)%、光周期L∶D=16h∶8h、光照强度8 250lx的人工气候箱中进行群体饲养,观察不同饲料配方对斜纹夜蛾生长发育的影响。【结果】供试4种饲料配方中,配方4(玉米粉200g,大豆粉100g,酵母粉12g,琼脂15g,抗坏血酸6g,山梨酸2g,对羟基苯甲酸甲酯2g,复配维生素液15mL,蔗糖50g,蒸馏水1 000mL)的饲养效果最好。用配方4饲养斜纹夜蛾幼虫,其幼虫到成虫发育历期最短,仅26.77d,幼虫存活率高达97%,蛹质量0.3g,雌雄性比达到0.91∶1,单雌产卵量为774粒。【结论】供试4种配方中以配方4的饲养效果最好,可以作为斜纹夜蛾室内饲养的半人工饲料。[Objective]The purpose of this paper was to improve the semi-artificial feed formula for cotton leafwormSpodoptera litura.[Method]Four different food formulas were designed and fed to S.litura under temperature of（27±0.5）℃,relative humidity of（70±5）%,photoperiod of 16h∶8h（L∶D）,and light intensity of 8 250 lx in climate-control chamber.Then,the effects of different formulas on growth of cotton leafworm were observed.[Result]Formula 4（maize powder 200 g,soybean powder 100 g,yeast powder 12 g,agar 15 g,L-ascorbic acid 6g,sorbic acid 2g,nipagin ester 2g,vitamins liquid 15 mL,saccharose 50 g and de-ionized water 1 000mL）showed the best desired effects.The cotton leafworm fed on formula 4had the shortest developmental duration（26.77d）from larva to adults,the highest survival rate（97%）,fresh weighty per pupa（0.3g）,the highest ratio of female to male（0.91）,and and the highest fecundity（774eggs per female）.[Conclusion]Formula 4was the best semi-artificial diet to culture cotton leafworm indoor.

关 键 词：斜纹夜蛾 人工饲养 饲料配方 半人工饲料 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]