应用RT-PCR和dot-ELISA方法检测单头灰飞虱体内水稻条纹病毒  

Detection of Rice stripe virus in individual small brown planthoppers by RT-PCR and dot-ELISA

作　　者：张水英[1] 蒋春和[2] 兰平秀[1,3] 张雪峰[4] 李凡[1] 

机构地区：[1]云南农业大学农业生物多样性与病虫害控制教育部重点实验室,昆明650201 [2]云南农业大学教务处,昆明650201 [3]云南农业大学动物科学技术学院,昆明650201 [4]红河学院,蒙自661199

出　　处：《植物保护》2016年第2期119-122,共4页Plant Protection

基　　金：云南省教育厅科学研究基金重大专项(ZD2012005);云南省高校科技创新团队支持计划(云教科[2014]22号)

摘　　要：本文比较了RT-PCR和dot-ELISA两种方法对单头灰飞虱携带水稻条纹病毒（Rice stripe virus,RSV）的检出率、检测灵敏度和检测成本。结果表明,两种方法均能检测到单头灰飞虱体内的RSV,RT-PCR检测单头灰飞虱体内RSV的阳性检出率比dot-ELISA方法低11.79%-15.77%,但RT-PCR的检测灵敏度比dot-ELISA高10倍,RT-PCR技术的检测成本比dot-ELISA的高。结果暗示,对于单头介体昆虫中的病毒检测,RT-PCR技术的检出率不一定比dot-ELISA的高。综合考虑后,如对室外大批量灰飞虱进行带毒率检测时可采用dot-ELISA方法,如需准确分析单头灰飞虱体内RSV时可采用RT-PCR方法。The detection rates,sensitivities and cost of RT-PCR and dot-ELISA methods were compared for the detection of Rice stripe virus（RSV）in individual small brown planthoppers（SBPH）.The results indicated that RSV could be detected in single SBPH successfully by both methods.The RSV-positive detection rates in single SBPHs by RT-PCR were 11.79%-15.77%lower than that by dot-ELISA;however,the sensitivity of RT-PCR was 10 times higher than that of dot-ELISA.The cost of RT-PCR was also higher.The results suggested that the detection rate of RSV in individual insect vectors by RT-PCR might be lower than that by dot-ELISA.Therefore,dot-ELISA is recommended for the detection of RSV in individual SBPHs when large-scale field samples need to be tested,while RT-PCR is used for accurate analysis of the virus in single SBPHs.

关 键 词：水稻条纹病毒 灰飞虱 RT-PCR DOT-ELISA 检测 

分 类 号：Q93-331[生物学—微生物学] S435.111.42[农业科学—农业昆虫与害虫防治]