国外引进甘蔗材料白叶病植原体巢式PCR检测及其序列分析  

Nested PCR detection and sequence analysis of phytoplasma causing white leaf disease in imported sugarcane materials

作　　者：王晓燕[1] 李文凤[1] 黄应昆 张荣跃[1] 单红丽[1] 

机构地区：[1]云南省农业科学院甘蔗研究所,云南省甘蔗遗传改良重点实验室,开远661699

出　　处：《植物保护》2016年第2期142-145,共4页Plant Protection

基　　金：国家现代农业产业技术体系(CARS-20-2-2);云南省现代农业产业技术体系

摘　　要：为有效防止引起重要危险性检疫病害甘蔗白叶病（SCWL）的植原体病原随引进甘蔗材料侵入我国,确保我国甘蔗生产安全,本研究对从缅甸、菲律宾、法国和泰国引进的22个甘蔗材料进行了SCWL植原体巢式PCR检测,并对阳性样品的巢式PCR产物进行了测序及序列分析。结果表明,18个甘蔗材料呈SCWL植原体阳性,阳性检出率为81.8%。所有SCWL阳性样品的16S-23S基因间隔区片段长210bp,与GenBank中已有的其他SCWL植原体分离物（登录号HQ917068、AB646271）的同源性为99.8%-100%,并在系统发育树中聚为一个类群。根据SCWL的巢氏PCR检测及其序列分析结果,对呈阳性的材料及时进行了集中销毁处理。To effectively prevent invasion of sugarcane white leaf disease（SCWL）,an important quarantine disease caused by phytoplasma,into China through introduction of sugarcane materials,and to secure sugarcane production,phytoplasma causing SCWL was detected by nested PCR in22 imported sugarcane materials from Myanmar,the Philippines,France and Thailand.Then,the nested PCR products of SCWL positive samples were sequenced and analyzed.The results showed that 18 of 22 materials（81.8%）were positive.The 16S-23 S intergenic region of all SCWL positive samples were all 210 bp long,and shared 99.8% to 100% identity with other published SCWL phytoplasma sequences（GenBank accession number HQ917068,AB646271）,and they were clustered together in a group in phylogenetic tree.Based on the nested PCR detection and sequence analysis results,the SCWL positive materials were destroyed immediately.

关 键 词：国外甘蔗材料 甘蔗白叶病 巢式PCR检测 序列分析 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治] Q939.34[农业科学—植物保护]