大鼠失肌皮神经与屈肘重建后供受体肌内亚型乙酰胆碱受体的表达  

Expression of subtypes acetylcholine receptors in donor and recipient muscles after musculocutaneous nerve transection and reconstruction of elbow flexion in rats

作　　者：易晓东[1] 杨胜波[2] 

机构地区：[1]遵义医学院组胚教研室,贵州遵义563003 [2]遵义医学院解剖学教研室,贵州遵义563003

出　　处：《中国临床解剖学杂志》2016年第2期180-185,共6页Chinese Journal of Clinical Anatomy

基　　金：贵州省科技厅社会发展攻关项目(黔科合SY字2012-3120)

摘　　要：目的探索失肌皮神经与胸大肌部分移位重建屈肘后供受体肌内成熟型与胚胎型乙酰胆碱受体(ε-ACh R和γ-ACh R)的表达变化,旨在为其术后肌萎缩的防治提供新视角。方法将70只成年SD大鼠随机分为A组(正常组);B1、B2组(肌皮神经切断2、4周组);C1、C2组(假移植术2、4周组);D1、D2组(胸大肌部分移位缝合至肱二头肌腱重建屈肘功能术2、4周组),共7组,每组10只。称量肌重,HE染色后测量肌纤维横切面积,RT-PCR和Western blot技术检测ε-ACh R和γ-ACh R的m RNA与蛋白表达变化。结果与A组比较,B组大鼠跛行,觅食受限,肱二头肌(受体肌)湿重逐渐减轻,肌纤维横切面积减小,ε-ACh R m RNA和蛋白表达下调,γ-ACh R出现重新表达并逐渐上调;C组中上述改变进行性加重,D组的上述改变逐渐恢复,且胸大肌(供体肌)内ε和γ-ACh R仅一过性异常表达。结论大鼠失肌皮神经损伤后,行胸大肌部分移位重建屈肘功能术,能上调受体肌内ε-ACh R表达和下调γ-ACh R的表达而改善屈肘功能。Objective To explore the expression changes of acetylcholinereceptors（ACh R）ε and γsubtypes（ε- ACh R and γ- ACh R） in the recipient muscle（biceps brachii） and donor muscle（oblique part of pectoralis major） after musculocutaneous nerve transection and elbow flexion reconstruction, and to provide a scientific guidance for the prevention and treatment of donor and recipient muscles＇ atrophy. Methods Seventy adult Sprague Dawley rats were randomly divided into group A（normal）; groups B1 and B2（2w and4 w post- musculocutaneous nerve transection）; groups C1 and C2（2w and 4w post- false- reconstruction of elbow flexion）; groups D1 and D2（2w and 4w post- reconstruction of elbow flexion）; There were 7 animal groups, and each group had 10 rats. Muscle wet weights were assessed, muscle fiber cross-sectional area were measured after HE staining. ε- ACh R and γ- ACh R m RNA expressions were detected by RT- PCR. ε- ACh R and γ-ACh R protein expressions were detected by western blot technology. Results Compared with rats in group A, rats in group B became limp after musculocutaneous nerve transection, and the foraging movements were restricted, the weight of biceps brachii（recipient muscle） muscle shrunk gradually, the cross- sectional area decreased, the ε- ACh R m RNA and protein expressions were downregulated. The γ- ACh R expression reappeared later and followed an increasing trend gradually; However, the aboveme ntioned changes were far worse in group C and gradually improved in group D while expression of γ-ACh R and ε-ACh R in pectoralis major（donor muscle） showed only transient changes. Conclusions The transfer of the oblique part of the pectoralis major for elbow flexion reconstruction after musculocutaneous nerve transection can upregulate ε-ACh R and downregulate γ-ACh R in the recipient muscle and improve the function of elbow flexion in rats.

关 键 词：肌皮神经损伤 屈肘重建 成熟型乙酰胆碱受体(ε-AChR) 胚胎型乙酰胆碱受体(γ-AChR) 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]